Rabbit anti-Human, Mouse DTL Polyclonal Antibody | anti-DTL antibody

DTL Polyclonal Antibody

Cat. Num. AAA2562436

• Gene Names: DTL; CDT2; RAMP; DCAF2; L2DTL
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Antigen Affinity Purification
• Synonyms: DTL; Polyclonal Antibody; DTL Polyclonal Antibody; Lethal(2) denticleless protein homolog; CDW1; DCAF2; DDB1 and CUL4 associated factor 2; Ddb1- and Cul4-associated factor 2; Denticleless homolog; Denticleless homolog (Drosophila); Denticleless protein homolog; Dtl; L2DTL; RA regulated nuclear matrix associated protein; RAMP; Retinoic acid regulated nuclear matrix associated protein; Retinoic acid-regulated nuclear matrix-associated protein; anti-DTL antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
• Concentration: 0.6mg/mL (varies by lot)
• Sequence Length: 730

• Applicable Applications for anti-DTL antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:20-1:100; ELISA: 1:5000-1:10000
• Immunogen: Fusion protein of human DTL
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using DTL Polyclonal Antibody at dilution of 1:30(×200))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer tissue using DTL Polyclonal Antibody at dilution of 1:30(×200))

Related Product Information for anti-DTL antibody

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBXO18/FBH1 and KMT5A (PubMed:16861906, PubMed:16949367, PubMed:16964240, PubMed:17085480, PubMed:18703516, PubMed:18794347, PubMed:18794348, PubMed:19332548, PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed:16861906, PubMed:16949367, PubMed:17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed:18794348, PubMed:19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed:23478445). Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613).

Product Categories/Family for anti-DTL antibody

Cancer; Cell biology

NCBI and Uniprot Product Information

• NCBI GI #: 188219762
• NCBI GeneID: 51514
• NCBI Accession #: NP_057532.2; BC033540
• NCBI GenBank Nucleotide #: NP_057532.2
• UniProt Accession #: Q9NZJ0
• NCBI Official Full Name: denticleless protein homolog
• NCBI Official Synonym Full Names: denticleless E3 ubiquitin protein ligase homolog
• NCBI Official Symbol: DTL
• NCBI Official Synonym Symbols: CDT2; RAMP; DCAF2; L2DTL
• NCBI Protein Information: denticleless protein homolog
• UniProt Protein Name: Denticleless protein homolog
• Protein Family: Denticleless protein
• UniProt Gene Name: DTL
• UniProt Synonym Gene Names: CDT2; CDW1; DCAF2; L2DTL; RAMP
• UniProt Entry Name: DTL_HUMAN

Uniprot Description

RAMP: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. Belongs to the WD repeat cdt2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; centrosome; nuclear membrane; intracellular membrane-bound organelle; cytoplasm; nucleolus; chromosome; nucleus

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: protein monoubiquitination; ubiquitin-dependent protein catabolic process; bypass DNA synthesis; protein polyubiquitination; regulation of cell cycle; G2/M transition DNA damage checkpoint; DNA replication; response to DNA damage stimulus; response to UV