Mouse anti-Human Hexokinase Monoclonal Antibody | anti-HXKP antibody

Hexokinase, Type IV (HK IV, HKIV, Hexokinase-4, HK4, FGQTL3, Glucokinase, GCK, GK, GLK, Hexokinase-D, HHF3, HXKP, LGLK, MODY2) (PE)

Cat. Num. AAA6158174

• Gene Names: GCK; GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Hexokinase; Monoclonal Antibody; Type IV (HK IV; HKIV; Hexokinase-4; HK4; FGQTL3; Glucokinase; GCK; GK; GLK; Hexokinase-D; HHF3; HXKP; LGLK; MODY2) (PE); anti-HXKP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3C7
• Specificity: Recognizes human GCK.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HXKP antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa356-465 from human GCK (AAH01890.1) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: GLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.73kD).)

Testing Data

(Detection limit for recombinant GST tagged GCK is ~0.1ng/ml as a capture antibody.)

Related Product Information for anti-HXKP antibody

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non- insulin dependent diabetes mellitus (NIDDM), also called maturity onset diabetes of the young, type 2 (MODY2); mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Product Categories/Family for anti-HXKP antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 12804882
• NCBI GeneID: 2645
• Molecular Weight: 52,035 Da
• NCBI Official Full Name: Homo sapiens glucokinase (hexokinase 4), mRNA
• NCBI Official Synonym Full Names: glucokinase
• NCBI Official Symbol: GCK
• NCBI Official Synonym Symbols: GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
• NCBI Protein Information: glucokinase
• Protein Family: Hexokinase

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009]