Rabbit anti-Human WRN Polyclonal Antibody | anti-WRN antibody

WRN Polyclonal Antibody

Cat. Num. AAA2561469

• Gene Names: WRN; RECQ3; RECQL2; RECQL3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Antigen Affinity Purification
• Synonyms: WRN; Polyclonal Antibody; WRN Polyclonal Antibody; DKFZp686C2056; DNA helicase; RecQ like type 3; Exonuclease WRN; HGNC 12791; OTTHUMP00000225301; RecQ protein-like 2; RecQ-like type 3; RecQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; Werner syndrome helicase; Werner syndrome protein; Werner syndrome; RecQ helicase like; anti-WRN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
• Concentration: 0.4mg/mL (varies by lot)
• Sequence Length: 1432

• Applicable Applications for anti-WRN antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:25-1:100; ELISA: 1:5000-1:10000
• Immunogen: Synthetic peptide of human WRN
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer tissue using WRN Polyclonal Antibody at dilution of 1:30(×200))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human lung cancer tissue using WRN Polyclonal Antibody at dilution of 1:30(×200))

Related Product Information for anti-WRN antibody

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Product Categories/Family for anti-WRN antibody

Cancer; Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 110735439
• NCBI GeneID: 7486
• NCBI Accession #: NP_000544.2
• NCBI GenBank Nucleotide #: NP_000544.2
• UniProt Accession #: Q14191
• NCBI Official Full Name: Werner syndrome ATP-dependent helicase
• NCBI Official Synonym Full Names: WRN RecQ like helicase
• NCBI Official Symbol: WRN
• NCBI Official Synonym Symbols: RECQ3; RECQL2; RECQL3
• NCBI Protein Information: Werner syndrome ATP-dependent helicase
• UniProt Protein Name: Werner syndrome ATP-dependent helicase
• Protein Family: Werner syndrome ATP-dependent helicase
• UniProt Gene Name: WRN
• UniProt Synonym Gene Names: RECQ3; RECQL2; RecQ3
• UniProt Entry Name: WRN_HUMAN

NCBI Description

This gene encodes a member of the RecQ subfamily of DNA helicase proteins. The encoded nuclear protein is important in the maintenance of genome stability and plays a role in DNA repair, replication, transcription and telomere maintenance. This protein contains a N-terminal 3' to 5' exonuclease domain, an ATP-dependent helicase domain and RQC (RecQ helicase conserved region) domain in its central region, and a C-terminal HRDC (helicase RNase D C-terminal) domain and nuclear localization signal. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by accelerated aging and an elevated risk for certain cancers. [provided by RefSeq, Aug 2017]

Uniprot Description

WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.

Protein type: Nucleolus; EC 3.6.4.12; DNA repair, damage; Helicase; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; MutLalpha complex; centrosome; nucleolus

Molecular Function: G-quadruplex DNA binding; protein homodimerization activity; ATPase activity; magnesium ion binding; 3'-5' DNA helicase activity; bubble DNA binding; helicase activity; four-way junction helicase activity; ATP-dependent DNA helicase activity; Y-form DNA binding; protein binding; DNA helicase activity; DNA binding; ATP-dependent 3'-5' DNA helicase activity; manganese ion binding; exonuclease activity; protein complex binding; 3'-5' exonuclease activity; ATP binding

Biological Process: nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; multicellular organismal aging; cell aging; cellular response to starvation; replicative cell aging; response to UV-C; DNA duplex unwinding; replication fork processing; DNA recombination; regulation of apoptosis; DNA synthesis during DNA repair; base-excision repair; double-strand break repair; regulation of growth rate; response to oxidative stress; DNA replication; response to DNA damage stimulus; DNA metabolic process; telomere maintenance; aging

Disease: Werner Syndrome