Erythropoietin R/EpoR Recombinant Protein | EpoR recombinant protein

Recombinant Human Erythropoietin R/EpoR Protein (C-Fc)

Cat. Num. AAA2557295

• Gene Names: EPOR; EPO-R
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Erythropoietin R/EpoR; Recombinant Human Erythropoietin R/EpoR Protein (C-Fc); EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358; Platelet Receptor EPO-R; EpoR recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: Ala25-Pro250
• Sequence Length: 508

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-Fc
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for EpoR recombinant protein

Protein Construction: Recombinant Human Erythropoietin R is produced by our mammalian expression system and the target gene encoding Ala25-Pro250 is expressed with a Fc tag at the C-terminus.

Background: Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the erythropoietin receptor (Epo R). The presence of a soluble form of the Epo R has also been detected on human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

NCBI and Uniprot Product Information

• NCBI GI #: 4503591
• NCBI GeneID: 2057
• NCBI Accession #: NP_000112.1
• NCBI GenBank Nucleotide #: NP_000112.1
• UniProt Accession #: P19235
• Molecular Weight: Molecular Mass: 54.5kDa; Actual Protein Molecular Mass: 55-75kDa
• NCBI Official Full Name: erythropoietin receptor
• NCBI Official Synonym Full Names: erythropoietin receptor
• NCBI Official Symbol: EPOR
• NCBI Official Synonym Symbols: EPO-R
• NCBI Protein Information: erythropoietin receptor
• UniProt Protein Name: Erythropoietin receptor
• Protein Family: Erythropoietin receptor
• UniProt Gene Name: EPOR
• UniProt Synonym Gene Names: EPO-R
• UniProt Entry Name: EPOR_HUMAN

NCBI Description

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: identical protein binding; protein binding; erythropoietin receptor activity

Biological Process: heart development; brain development; decidualization; signal transduction

Disease: Erythrocytosis, Familial, 1