HAI-2/SPINT2 recombinant protein

Recombinant Human HAI-2/SPINT2 Protein (C-His)

Cat. Num. AAA2557267

• Gene Names: SPINT2; PB; Kop; HAI2; DIAR3; HAI-2
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: HAI-2/SPINT2; Recombinant Human HAI-2/SPINT2 Protein (C-His); Kunitz-Type Protease Inhibitor 2; Hepatocyte Growth Factor Activator Inhibitor Type 2; HAI-2; Placental Bikunin; SPINT2; HAI2; KOP; HAI-2/SPINT2 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH7.4.
• Sequence: Ala28-Lys197
• Sequence Length: 195

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for HAI-2/SPINT2 recombinant protein

Protein Construction: Recombinant Human Hepatocyte Growth Factor Activator Inhibitor Type 2 is produced by our mammalian expression system and the target gene encoding Ala28-Lys197 is expressed with a 6His tag at the C-terminus.

Background: Hepatocyte Growth Factor Activator Inhibitor Type 2 (HAI2) is a single-pass type I membrane protein and contains two BPTI/Kunitz inhibitor domains. The first Kunitz domain is mainly responsible for the inhibitory activity against hepatocyte growth factor activator (HGFA). HAI2 is expressed in placenta, kidney, pancreas, prostate, testis, thymus and trachea. HAI2 serves as a inhibitor of HGF activator. It also inhibits plasmin, plasma and tissue kallikrein and factor XIa. Defects in HAI2 are the cause of diarrhea type 3 (DIAR3), also known as congenital sodium diarrhea (CSD).

NCBI and Uniprot Product Information

• NCBI GI #: 260656008
• NCBI GeneID: 10653
• NCBI Accession #: NP_001159575.1
• NCBI GenBank Nucleotide #: NP_001159575.1
• UniProt Accession #: O43291
• Molecular Weight: Molecular Mass: 20.22kDa; Actual Protein Molecular Mass: 24-30kDa
• NCBI Official Full Name: kunitz-type protease inhibitor 2 isoform b
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kunitz type 2
• NCBI Official Symbol: SPINT2
• NCBI Official Synonym Symbols: PB; Kop; HAI2; DIAR3; HAI-2
• NCBI Protein Information: kunitz-type protease inhibitor 2
• UniProt Protein Name: Kunitz-type protease inhibitor 2
• UniProt Gene Name: SPINT2
• UniProt Synonym Gene Names: HAI2; KOP; HAI-2
• UniProt Entry Name: SPIT2_HUMAN

NCBI Description

This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

SPINT2: Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa. Defects in SPINT2 are the cause of diarrhea type 3 (DIAR3); also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm; extracellular region

Molecular Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: cell motility; negative regulation of cell-cell adhesion

Disease: Diarrhea 3, Secretory Sodium, Congenital, With Or Without Other Congenital Anomalies