Mouse anti-Human CD8 Monoclonal Antibody | anti-CD8 antibody

Anti-Human CD8 Monoclonal Antibody (Alexa Fluor 647 Conjuaged)

Cat. Num. AAA2556916

• Gene Names: CD8A; CD8; p32; Leu2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD8; Monoclonal Antibody; Anti-Human CD8 Monoclonal Antibody (Alexa Fluor 647 Conjuaged); T-cell surface glycoprotein CD8 alpha chain; CD8A; T-lymphocyte differentiation antigen T8/Leu-2; MAL; anti-CD8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: OKT-8
• Form/Format: Liquid; PBS with 0.05% Proclin300, 1% BSA
• Sequence Length: 235

• Applicable Applications for anti-CD8 antibody: Flow Cytometry (FC/FACS)
• Conjugation: Alexa Fluor 647
• Preparation and Storage: Store at 2-8 degree C and protected from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(Human peripheral blood lymphocytes are stained with Anrti-Human CD8 Monoclonal Antibody(Alexa Fluor 647 Conjuaged)(filled gray histogram). Unstained lymphocytes (empty black histogram) are used as control.)

Related Product Information for anti-CD8 antibody

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the alpha3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

References

1. Knapp W. W. Knapp.. et al.Oxford University Press; 1989; :1-1182. 2. Reimann KA, Waite BC, Lee-Parritz DE, et al. Cytometry. 1994; 17(1):102-108. 3. Roederer M, Kantor AB, Parks DR, Herzenberg LA. Cytometry. 1996; 24(3):191-197. 4. Schlossman SF. Stuart F. Schlossman.. et al. Oxford University Press; 1995. 5. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Cytometry. 1997; 29(4):351-362.

NCBI and Uniprot Product Information

• NCBI GI #: 225007536
• NCBI GeneID: 925
• NCBI Accession #: NP_001139345.1
• NCBI GenBank Nucleotide #: NM_001145873.1
• UniProt Accession #: P01732
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: CD8A
• NCBI Official Synonym Symbols: CD8; p32; Leu2
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: CD81 antigen
• UniProt Gene Name: CD8A
• UniProt Synonym Gene Names: MAL
• UniProt Entry Name: CD8A_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]