Rabbit anti-Mouse, Rat DLL1 Polyclonal Antibody | anti-DLL1 antibody

DLL1 Polyclonal Antibody

Cat. Num. AAA2556206

• Gene Names: Dll1; Delta1
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: DLL1; Polyclonal Antibody; DLL1 Polyclonal Antibody; Delta (Drosophila) like 1; Delta; Delta like 1 (Drosophila); Delta like 1; Delta like 1 protein; Delta like protein 1; Delta like protein 1 precursor; Drosophila; homolog of 1; Delta-like protein 1; Delta1; DL1; DLL 1; Drosophila Delta homolog 1; H-Delta-1; OTTHUMP00000017690; anti-DLL1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 100 mug/ml BSA and 50% glycerol.
• Concentration: 400 mug/mL (varies by lot)
• Sequence Length: 722

• Applicable Applications for anti-DLL1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:200-1:500
• Immunogen: KLH conjugated synthetic peptide corresponding to Mouse DLL1
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded mouse brain using DLL1 Polyclonal Antibody at dilution of 1:200.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded rat spinal marrow using DLL1 Polyclonal Antibody at dilution of 1:200.)

Related Product Information for anti-DLL1 antibody

DLL1 is a homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis.

Product Categories/Family for anti-DLL1 antibody

Neuroscience; Epigenetics and Nuclear Signaling; Stem Cells; Developmental Biology

NCBI and Uniprot Product Information

• NCBI GI #: 40789272
• NCBI GeneID: 13388
• NCBI Accession #: NP_031891.2
• NCBI GenBank Nucleotide #: NM_007865.3
• UniProt Accession #: Q61483; Q6PFV7
• Molecular Weight: 78,449 Da
• NCBI Official Full Name: delta-like protein 1
• NCBI Official Synonym Full Names: delta like canonical Notch ligand 1
• NCBI Official Symbol: Dll1
• NCBI Official Synonym Symbols: Delta1
• NCBI Protein Information: delta-like protein 1
• UniProt Protein Name: Delta-like protein 1
• Protein Family: Delta-like protein
• UniProt Gene Name: Dll1
• UniProt Synonym Gene Names: Shed form1 PublicationManual assertion based on opinion iniRef.6; Dll1-TMIC1 PublicationManual assertion inferred by curator fromiRef.6; Membrane-associated fragment1 PublicationManual assertion based on opinion iniRef.6; Dll1-IC1 PublicationManual assert

Uniprot Description

Transmembrane ligand protein of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain (ECD) of Notch receptor in a cis and trans fashion manner (PubMed:21985982, PubMed:10958687). Following transinteraction, ligand cells produce mechanical force that depends of a clathrin-mediated endocytosis, requiring ligand ubiquitination, EPN1 interaction, and actin polymerisation; these events promote Notch receptor extracellular domain (NECD) transendocytosis and triggers Notch signaling through induction of cleavage, hyperphosphorylation, and nuclear accumulation of the intracellular domain of Notch receptors (NICD) (PubMed:10958687, PubMed:18676613). Is required for embryonic development and maintenance of adult stem cells in many different tissues and immune systeme; the DLL1-induced Notch signaling is mediated through an intercellular communication that regulates cell lineage, cell specification, cell patterning and morphogenesis through effects on differentiation and proliferation (PubMed:17194759, PubMed:19562077, PubMed:18997111, PubMed:23695674, PubMed:16495313, PubMed:21238454, PubMed:22282195, PubMed:7671806, PubMed:17960184, PubMed:22529374, PubMed:19389377, PubMed:23699523, PubMed:19144989, PubMed:23688253, PubMed:23806616, PubMed:26114479, PubMed:22940113, PubMed:25220152, PubMed:20081190, PubMed:21572390, PubMed:22096075). Plays a role in brain development at different level, namely by regulating neuronal differentiation of neural precursor cells via cell-cell interaction, most likely through the lateral inhibitory system in an endogenous level dependent-manner (PubMed:7671806, PubMed:18997111). During neocortex development, Dll1-Notch signaling transmission is mediated by dynamic interactions between intermediate neurogenic progenitors and radial glia; the cell-cell interactions are mediated via dynamic and transient elongation processes, likely to reactivate/maintain Notch activity in neighboring progenitors, and coordinate progenitor cell division and differentiation across radial and zonal boundaries (PubMed:23699523). During cerebellar development, regulates Bergmann glial monolayer formation and its morphological maturation through a Notch signaling pathway (PubMed:23688253). At the retina and spinal cord level, regulates neurogenesis by preventing the premature differentiation of neural progenitors and also by maintaining progenitors in spinal cord through Notch signaling pathway (PubMed:19389377, PubMed:26114479). Also controls neurogenesis of the neural tube in a progenitor domain-specific fashion along the dorsoventral axis (PubMed:20081190). Maintains quiescence of neural stem cells and plays a role as a fate determinant that segregates asymmetrically to one daughter cell during neural stem cells mitosis, resulting in neuronal differentiation in Dll1-inheriting cell (PubMed:23695674). Plays a role in immune systeme development, namely the development of all T-cells and marginal zone (MZ) B cells (PubMed:15146182, PubMed:19217325). Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor (). Upon MMP14 cleavage, negatively regulates Notch signaling in haematopoietic progenitor cells to specifically maintain normal B-cell development in bone marrow (PubMed:21572390). Also plays a role during muscle development. During early development, inhibits myoblasts differentiation from the medial dermomyotomal lip and later regulates progenitor cell differentiation (PubMed:17194759). Directly modulates cell adhesion and basal lamina formation in satellite cells through Notch signaling. Maintains myogenic progenitors pool by suppressing differentiation through down-regulation of MYOD1 and is required for satellite cell homing and PAX7 expression (PubMed:22940113). During craniofacial and trunk myogenesis suppresses differentiation of cranial mesoderm-derived and somite-derived muscle via MYOD1 regulation but in cranial mesoderm-derived progenitors, is neither required for satellite cell homing nor for PAX7 expression (PubMed:25220152). Also plays a role during pancreatic cell development. During type B pancreatic cell development, may be involved in the initiation of proximodistal patterning in the early pancreatic epithelium (PubMed:22529374). Stimulates multipotent pancreatic progenitor cells proliferation and pancreatic growth by maintaining HES1 expression and PTF1A protein levels (PubMed:22096075). During fetal stages of development, is required to maintain arterial identity and the responsiveness of arterial endothelial cells for VEGFA through regulation of KDR activation and NRP1 expression (PubMed:19144989). Controls sprouting angiogenesis and subsequent vertical branch formation througth regulation on tip cell differentiation (PubMed:22282195). Negatively regulates goblet cell differentiation in intestine and controls secretory fat commitment through lateral inhibition in small intestine (PubMed:21238454, PubMed:21915337). Plays a role during inner ear development; negatively regulates auditory hair cell differentiation (PubMed:16495313). Plays a role during nephron development through Notch signaling pathway (PubMed:23806616). Regulates growth, blood pressure and energy homeostasis (PubMed:19562077).