Rabbit anti-Human, Mouse Ku-70 Polyclonal Antibody | anti-Ku-70 antibody

Phospho-Ku-70 (Ser5) Polyclonal Antibody

Cat. Num. AAA2548835

• Gene Names: XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity Purification
• Synonyms: Ku-70; Polyclonal Antibody; Phospho-Ku-70 (Ser5) Polyclonal Antibody; XRCC6; G22P1; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-; anti-Ku-70 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 609

• Applicable Applications for anti-Ku-70 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:100-1:300; ELISA: 1:5000
• Immunogen: Synthesized peptide derived from human Ku-70 around the phosphorylation site of Ser5
• Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Rat kidney, HepG2-UV with Phospho-Ku-70 (Ser5) Polyclonal Antibody at dilution of 1:1000)

Related Product Information for anti-Ku-70 antibody

Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.

Product Categories/Family for anti-Ku-70 antibody

Acetyl/Phospho/Methyl Antibody; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 573014815
• NCBI GeneID: 2547
• NCBI Accession #: NP_001275905.1
• NCBI GenBank Nucleotide #: NM_001288976.1
• UniProt Accession #: P12956; Q6FG89; Q9UCQ2; Q9UCQ3; B1AHC8
• Molecular Weight: Calculated Molecular Weight: 70kDa; Observed Molecular Weight: 70kDa
• NCBI Official Full Name: X-ray repair cross-complementing protein 6 isoform 1
• NCBI Official Synonym Full Names: X-ray repair cross complementing 6
• NCBI Official Symbol: XRCC6
• NCBI Official Synonym Symbols: ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• NCBI Protein Information: X-ray repair cross-complementing protein 6
• UniProt Protein Name: X-ray repair cross-complementing protein 6
• UniProt Gene Name: XRCC6
• UniProt Synonym Gene Names: G22P1; 5'-dRP lyase Ku70; CTC75; CTCBF; Ku70; TLAA

NCBI Description

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Uniprot Description

Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: DNA repair, damage; DNA-binding; EC 3.6.4.-; EC 4.2.99.-; Helicase; Nuclear receptor co-regulator; RNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: cytosol; extracellular region; ficolin-1-rich granule lumen; membrane; nonhomologous end joining complex; nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleolus; nucleoplasm; nucleus; protein complex; secretory granule lumen; transcription factor complex

Molecular Function: 5'-deoxyribose-5-phosphate lyase activity; ATP binding; ATP-dependent DNA helicase activity; cyclin binding; damaged DNA binding; DNA binding; double-stranded DNA binding; double-stranded telomeric DNA binding; protein binding; protein C-terminus binding; RNA binding; transcription regulatory region DNA binding

Biological Process: activation of innate immune response; brain development; cellular hyperosmotic salinity response; cellular response to gamma radiation; cellular response to X-ray; DNA duplex unwinding; DNA ligation; DNA recombination; double-strand break repair via nonhomologous end joining; innate immune response; negative regulation of transcription, DNA-dependent; neutrophil degranulation; positive regulation of interferon type I production; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; protein heterotetramerization; regulation of smooth muscle cell proliferation; telomere maintenance; transcription, DNA-dependent