Western Blot (WB)
(Western Blot analysis of 22RV1 cells with Phospho-VCP (Ser352) Polyclonal Antibody at dilution of 1:1000)
Rabbit VCP Polyclonal Antibody | anti-VCP antibody
Phospho-VCP (Ser352) Polyclonal Antibody
ELISA: 1:5000
Western Blot (WB)
(Western Blot analysis of 22RV1 cells with Phospho-VCP (Ser352) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of 22RV1 cells with Phospho-VCP (Ser352) Polyclonal Antibody at dilution of 1:1000)
NCBI and Uniprot Product Information
Observed Molecular Weight: 85kDa
NCBI Description
This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients. [provided by RefSeq, Aug 2017]
Uniprot Description
VCP: an ATPase enzyme present in all eukaryotes and archaebacteria that functions involved in vesicle transport and fusion, and 26S proteasomal degradation. Functions to extract protein molecules from large protein assemblies, membranes and chromatin, facilitating the proteasomal degradation of the extracted proteins by the proteasome. Involved in: the fragmentation of Golgi stacks during mitosis, and for their reassembly after mitosis; the formation of the transitional endoplasmic reticulum (tER); spindle disassembly at the end of mitosis; and the formation of a closed nuclear envelope. Involved in endoplasmic reticulum stress-induced preemptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Acts as a negative regulator of type I interferon production by interacting with DDX58: interaction takes place when DDX58 is ubiquitinated via 'Lys-63'-linked ubiquitin on its CARD domains, leading to recruit RNF125 and promote ubiquitination and degradation of DDX58. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization.
Protein type: Chaperone; DNA repair, damage; EC 3.6.4.6; Endoplasmic reticulum; Hydrolase
Chromosomal Location of Human Ortholog: 9p13.3
Cellular Component: ATPase complex; cytoplasm; cytosol; Derlin-1 retrotranslocation complex; endoplasmic reticulum; endoplasmic reticulum membrane; extracellular exosome; extracellular region; ficolin-1-rich granule lumen; intracellular membrane-bound organelle; lipid droplet; myelin sheath; nucleoplasm; nucleus; perinuclear region of cytoplasm; proteasome complex; protein complex; secretory granule lumen; VCP-NSFL1C complex
Molecular Function: ADP binding; ATP binding; ATPase activity; BAT3 complex binding; identical protein binding; K48-linked polyubiquitin modification-dependent protein binding; lipid binding; MHC class I protein binding; polyubiquitin modification-dependent protein binding; protein binding; protein domain specific binding; protein phosphatase binding; RNA binding; ubiquitin protein ligase binding; ubiquitin-like protein ligase binding; ubiquitin-specific protease binding
Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; ATP metabolic process; autophagy; bypass DNA synthesis; cellular response to DNA damage stimulus; DNA repair; double-strand break repair; endoplasmic reticulum stress-induced pre-emptive quality control; endoplasmic reticulum unfolded protein response; endosome to lysosome transport via multivesicular body sorting pathway; ER to Golgi vesicle-mediated transport; ER-associated misfolded protein catabolic process; ER-associated protein catabolic process; ERAD pathway; error-free translesion synthesis; establishment of protein localization; flavin adenine dinucleotide catabolic process; macroautophagy; NADH metabolic process; neutrophil degranulation; positive regulation of ATP biosynthetic process; positive regulation of mitochondrial membrane potential; positive regulation of oxidative phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; positive regulation of protein complex assembly; positive regulation of ubiquitin-specific protease activity; proteasomal protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid methylation; protein deubiquitination; protein folding; protein homooligomerization; protein N-linked glycosylation via asparagine; protein ubiquitination; regulation of apoptosis; retrograde protein transport, ER to cytosol; transmembrane transport; viral genome replication
Disease: Amyotrophic Lateral Sclerosis 14, With Or Without Frontotemporal Dementia; Charcot-marie-tooth Disease, Axonal, Type 2y; Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 1
Research Articles on VCP
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Product Notes
The VCP vcp (Catalog #AAA2548831) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-VCP (Ser352) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VCP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:5000. Researchers should empirically determine the suitability of the VCP vcp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VCP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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