IL4R / Il4ra recombinant protein

Recombinant Rat IL4R / Il4ra Protein (His tag)

Cat. Num. AAA2547428

• Gene Names: Il4r; Il4ra
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: IL4R / Il4ra; Recombinant Rat IL4R / Il4ra Protein (His tag); IL4R; Il4ra; IL4R / Il4ra recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 801

• Application Notes: The recombinant rat IL4R is a disulfide-linked homodimer. The reduced monomer comprises 218 amino acids and predicts a molecular mass of 25.3 kDa. The apparent molecular mass of the recombinant protein is approximately 46 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Ile 26
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for IL4R / Il4ra recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a type I transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.

Description: A DNA sequence encoding the rat IL4R (Q63257-1) (Met1-Arg232) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 158534071
• NCBI GeneID: 25084
• NCBI Accession #: NP_596871.2
• NCBI GenBank Nucleotide #: NM_133380.2
• UniProt Accession #: Q63257; Q9R1W8; Q9WTM8
• Molecular Weight: 26,001 Da
• NCBI Official Full Name: interleukin-4 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 4 receptor
• NCBI Official Symbol: Il4r
• NCBI Official Synonym Symbols: Il4ra
• NCBI Protein Information: interleukin-4 receptor subunit alpha
• UniProt Protein Name: Interleukin-4 receptor subunit alpha
• UniProt Gene Name: Il4r
• UniProt Synonym Gene Names: Il4ra; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA

NCBI Description

a receptor for the cytokine IL-4 [RGD, Feb 2006]

Uniprot Description

IL4R: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Belongs to the type I cytokine receptor family. Type 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1q36

Cellular Component: extracellular space; receptor complex

Molecular Function: protein heterodimerization activity

Biological Process: defense response to protozoan; immune response; immunoglobulin mediated immune response; inflammatory response; negative regulation of T-helper 1 cell differentiation; ovulation; positive regulation of immunoglobulin production; positive regulation of macrophage activation; positive regulation of mast cell degranulation; positive regulation of T-helper 2 cell differentiation; regulation of cell proliferation; response to estrogen stimulus