SDS-PAGE
JAM-2 / JAM-B recombinant protein
Recombinant Rat JAM-2 / JAM-B Protein (His tag)
Purity
> 90 % as determined by SDS-PAGE
Synonyms
JAM-2 / JAM-B; Recombinant Rat JAM-2 / JAM-B Protein (His tag); JAM2; JAM-2 / JAM-B recombinant protein
Host
Human Cells
Purity/Purification
> 90 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
298
Application Notes
The recombinant rat JAM2 comprises 219 amino acids and predicts a molecular mass of 24.7 kDa. The apparent molecular mass of the recombinant protein is approximately 43 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Predicted N Terminal
Phe 29
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for JAM-2 / JAM-B recombinant protein
Background: Junctional adhesion molecule B (JAM-B), also known as Junctional adhesion molecule 2 (JAM2), Vascular endothelial junction-associated molecule (VE-JAM), and CD322, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. It is prominently expressed on high endothelial venules. expression to be restricted to the high endothelial venule of tonsil and lymph nodes. The localization to the endothelium of arterioles in and around inflammatory and tumor foci. JAM-B can function as an adhesive ligand for the T cell line J45 and can interact with GM-CSF/IL-4-derived peripheral blood dendritic cells, circulating CD56 (+) NK cells, circulating CD56 (+)CD3 (+) NK/T cells, and circulating CD56 (+)CD3 (+)CD8 (+) cytolytic T cells. JAM-2 is expressed on high endothelial venules (HEVs) in human tonsil and on a subset of human leukocytes, suggesting that JAM-2 plays a central role in the regulation of transendothelial migration. It binds to very late activation antigen (VLA)-4, a leucocyte integrin that contributes to rolling and firm adhesion of lymphocytes to endothelial cells through binding to vascular cell adhesion molecule (VCAM)-1. JAM-B appears to contribute to leucocyte extravasation by facilitating not only transmigration but also rolling and adhesion. JAM-B acts as an adhesive ligand for interacting with a variety of immune cell type s and may play a role in lymphocyte homing to secondary lymphoid organs.
Description: A DNA sequence encoding the rat JAM2 (Q3MHC0) (Met1-Asn236) was expressed, fused with a polyhistidine tag at the C-terminus.
Description: A DNA sequence encoding the rat JAM2 (Q3MHC0) (Met1-Asn236) was expressed, fused with a polyhistidine tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,058 Da
NCBI Official Full Name
junctional adhesion molecule B
NCBI Official Synonym Full Names
junctional adhesion molecule 2
NCBI Official Symbol
Jam2
NCBI Protein Information
junctional adhesion molecule B
UniProt Protein Name
Junctional adhesion molecule 2
UniProt Gene Name
Jam2
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Product Notes
The JAM-2 / JAM-B jam2 (Catalog #AAA2547313) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant rat JAM2 comprises 219 amino acids and predicts a molecular mass of 24.7 kDa. The apparent molecular mass of the recombinant protein is approximately 43 kDa in SDS-PAGE under reducing conditions due to glycosylation. Researchers should empirically determine the suitability of the JAM-2 / JAM-B jam2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAM-2 / JAM-B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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