Carboxypeptidase B2 / CPB2 Recombinant Protein | CPB2 recombinant protein
Rat Carboxypeptidase B2 / CPB2 Protein (His Tag)
Purity
> 95 % as determined by SDS-PAGE.
Synonyms
Carboxypeptidase B2 / CPB2; Rat Carboxypeptidase B2 / CPB2 Protein (His Tag); CPB2; CPB2 recombinant protein
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE.
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
422
Application Notes
The recombinant rat Cpb2 consists of 412 amino acids and predicts a molecular mass of 48 kDa.
Predicted N Terminal
Phe 22
Endotoxin
< 1.0 EU per mug protein as determined by the LAL method.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
Related Product Information for CPB2 recombinant protein
Background: Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin / thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.
Description: A DNA sequence encoding the rat Cpb2 (NP_446069.1) (Met1-Ser422) was expressed with a polyhistidine tag at the C-terminus.
Description: A DNA sequence encoding the rat Cpb2 (NP_446069.1) (Met1-Ser422) was expressed with a polyhistidine tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,827 Da
NCBI Official Full Name
carboxypeptidase B2
NCBI Official Synonym Full Names
carboxypeptidase B2
NCBI Official Symbol
Cpb2
NCBI Protein Information
carboxypeptidase B2
UniProt Protein Name
Carboxypeptidase B2
Protein Family
UniProt Gene Name
Cpb2
UniProt Synonym Gene Names
CPR; CPU; TAFI
NCBI Description
a thrombin-activatable fibrinolysis inhibitor [RGD, Feb 2006]
Uniprot Description
CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.17.20; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 15q11
Cellular Component: extracellular space
Molecular Function: carboxypeptidase activity; metallocarboxypeptidase activity
Biological Process: fibrinolysis; negative regulation of fibrinolysis; proteolysis; response to drug; response to heat
Research Articles on CPB2
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Product Notes
The CPB2 cpb2 (Catalog #AAA2547265) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant rat Cpb2 consists of 412 amino acids and predicts a molecular mass of 48 kDa. Researchers should empirically determine the suitability of the CPB2 cpb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carboxypeptidase B2 / CPB2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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