MEK1 / MAP2K1 / MKK1 recombinant protein

Recombinant Mouse MEK1 / MAP2K1 / MKK1 Protein (His & GST tag)

Cat. Num. AAA2547046

• Gene Names: Map2k1; Mek1; MEKK1; MAPKK1; Prkmk1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: MEK1 / MAP2K1 / MKK1; Recombinant Mouse MEK1 / MAP2K1 / MKK1 Protein (His & GST tag); MAPKK1; MEKK1; Mek1; Prkmk1; MEK1 / MAP2K1 / MKK1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0
• Sequence Length: 393

• Application Notes: The recombinant mouse MAP2K1/GST chimera consists of 630 amino acids and has a calculated molecular mass of 71.3 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for MEK1 / MAP2K1 / MKK1 recombinant protein

Background: MEK1, also known as MAP2K1 and MKK1, is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Binding extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause cardiofaciocutaneous syndrome.

Description: A DNA sequence encoding the mouse MAP2K1 (P31938) (Met 1-Ile 393) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6678794
• NCBI GeneID: 26395
• NCBI Accession #: NP_032953.1
• NCBI GenBank Nucleotide #: NM_008927.3
• UniProt Accession #: P31938
• Molecular Weight: 43,474 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: Map2k1
• NCBI Official Synonym Symbols: Mek1; MEKK1; MAPKK1; Prkmk1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• UniProt Gene Name: Map2k1
• UniProt Synonym Gene Names: Mek1; Prkmk1; MAP kinase kinase 1; MAPKK 1; MEK 1

Uniprot Description

MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 9 C|9 34.55 cM

Cellular Component: axon; cell cortex; cytoplasm; cytosol; dendrite; dendrite cytoplasm; early endosome; endoplasmic reticulum; focal adhesion; Golgi apparatus; late endosome; microtubule; mitochondrion; nucleus; perikaryon; perinuclear region of cytoplasm; plasma membrane

Molecular Function: ATP binding; MAP kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; protein C-terminus binding; protein kinase binding; protein N-terminus binding; protein serine/threonine kinase activator activity; protein serine/threonine/tyrosine kinase activity; Ras GTPase binding; receptor signaling protein serine/threonine kinase activity; receptor signaling protein tyrosine phosphatase activity

Biological Process: activation of MAPK activity; Bergmann glial cell differentiation; cell cycle arrest; cell motility involved in cell locomotion; cell proliferation; cerebellar cortex formation; Golgi inheritance; heart development; keratinocyte differentiation; MAPKKK cascade; melanosome transport; mitosis; negative regulation of cell proliferation; negative regulation of homotypic cell-cell adhesion; neurite morphogenesis; neuron differentiation; positive regulation of axonogenesis; positive regulation of cell differentiation; positive regulation of cell migration; positive regulation of GTPase activity; positive regulation of Ras protein signal transduction; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; protein heterooligomerization; regulation of axon regeneration; regulation of protein amino acid phosphorylation; regulation of stress-activated MAPK cascade; regulation of vascular smooth muscle contraction; response to axon injury; response to glucocorticoid stimulus; response to oxidative stress; thymus development; thyroid gland development; vesicle transport along microtubule