DDR2 Kinase / CD167b recombinant protein

Recombinant Mouse DDR2 Kinase / CD167b Protein (His tag)

Cat. Num. AAA2546925

• Gene Names: Ddr2; Ntrkr3; tyro10; AW495251
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: DDR2 Kinase / CD167b; Recombinant Mouse DDR2 Kinase / CD167b Protein (His tag); AW495251; Ntrkr3; tyro10; Tkt; DDR2 Kinase / CD167b recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 854

• Application Notes: The recombinant mouse DDR2 consists of 389 amino acids and has a predicted molecular mass of 44 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm DDR2 is approximately 80-90 kDa due to glycosylation.
• Predicted N Terminal: Lys 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for DDR2 Kinase / CD167b recombinant protein

Background: Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.

Description: A DNA sequence encoding the extracellular domain of mouse DDR2 (NP_072075.2) (Met 1-Arg 399) was expressed, with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 158508514
• NCBI GeneID: 18214
• NCBI Accession #: NP_072075.2
• NCBI GenBank Nucleotide #: NM_022563.2
• UniProt Accession #: Q62371; B2RSD7
• Molecular Weight: 96,482 Da
• NCBI Official Full Name: discoidin domain-containing receptor 2
• NCBI Official Synonym Full Names: discoidin domain receptor family, member 2
• NCBI Official Symbol: Ddr2
• NCBI Official Synonym Symbols: Ntrkr3; tyro10; AW495251
• NCBI Protein Information: discoidin domain-containing receptor 2
• UniProt Protein Name: Discoidin domain-containing receptor 2
• UniProt Gene Name: Ddr2
• UniProt Synonym Gene Names: Ntrkr3; Tkt; Tyro10; Discoidin domain receptor 2

Uniprot Description

DDR2: This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: DDR family; EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 1 H3|1 76.84 cM

Cellular Component: apical plasma membrane; focal adhesion

Molecular Function: collagen binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: biomineral formation; collagen fibril organization; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of osteoblast differentiation; positive regulation of protein kinase activity; positive regulation of transcription factor activity; protein amino acid autophosphorylation; regulation of bone mineralization; transmembrane receptor protein tyrosine kinase signaling pathway