beta-Catenin / CTNNB1 Recombinant Protein | CTNNB1 recombinant protein

Recombinant Mouse beta-Catenin / CTNNB1 Protein (His & GST tag)

Cat. Num. AAA2546892

• Gene Names: Ctnnb1; Bfc; Mesc; Catnb
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: beta-Catenin / CTNNB1; Recombinant Mouse beta-Catenin / CTNNB1 Protein (His & GST tag); CTNNB1; Catnb; CTNNB1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
• Sequence Length: 781

• Application Notes: The recombinant mouse CTNNB1/GST chimera consists of 1018 amino acids and has a calculated molecular mass of 113 kDa. The recombinant protein migrates as an approximately 115 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CTNNB1 recombinant protein

Background: beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.

Description: A DNA sequence encoding the mouse CTNNB1 (Q02248) (Met1-Leu781) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 260166642
• NCBI GeneID: 12387
• NCBI Accession #: NP_001159374.1
• NCBI GenBank Nucleotide #: NM_001165902.1
• UniProt Accession #: Q02248; Q922W1; Q9D335
• Molecular Weight: 85,471 Da
• NCBI Official Full Name: catenin beta-1
• NCBI Official Synonym Full Names: catenin (cadherin associated protein), beta 1
• NCBI Official Symbol: Ctnnb1
• NCBI Official Synonym Symbols: Bfc; Mesc; Catnb
• NCBI Protein Information: catenin beta-1
• UniProt Protein Name: Catenin beta-1
• Protein Family: Catenin
• UniProt Gene Name: Ctnnb1
• UniProt Synonym Gene Names: Catnb

NCBI Description

This gene encodes not only an important cytoplasmic component of the classical cadherin adhesion complex that forms the adherens junction in epithelia and mediates cell-cell adhesion in many other tissues but also a key signaling molecule in the canonical Wnt signaling pathway that controls cell growth and differentiation during both normal development and tumorigenesis. The gene product contains a central armadillo-repeat containing domain through which it binds the cytoplasmic tail of classical cadherins; meanwhile, it also binds alpha-catenin, which further links the cadherin complex to the actin cytoskeleton either directly or indirectly. Beta-catenin is therefore necessary for the adhesive function of classical cadherins. Another key function of this protein is to mediate the canonical Wnt signaling pathway and regulate gene transcription. Without Wnt signal, cytoplasmic beta-catenin that is not associated with the cadherin complex is quickly phosphorylated at the N-terminal Ser/Thr residues by the so called degradation complex containing axin, adenomatous polyposis coli (APC), casein kinase I, and GSK3B, then ubiquitylated by beta-TrCP, and degraded by the proteasome. However, in the presence of Wnt signal, the degradation complex is disrupted and the stabilized cytoplasmic beta-catenin translocates into the nucleus, where it binds various transcription factors and, together with these factors, regulates the transcription of many downstream genes. Mutations of this gene have been linked with various types of tumors. Alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

CTNNB1: a regulator of cell adhesion and a key downstream effector in the Wnt signaling pathway. Implicated early embryonic development and tumorigenesis. Phosphorylated and destabilized by CK1 and GSK-3beta. Stabilized cytoplasmic beta-catenin is a hallmark of a variety of cancers. Stabilized beta-catenin translocates to the nucleus, where it acts as a transcriptional activator of T-cell factor (TCF)-regulated genes. Interacts with the PDZ domain of TAX1BP3, inhibiting its transcriptional activity. Two alternatively spliced human isoforms have been described.

Protein type: Actin-binding; Cell adhesion; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 9 F4|9 72.19 cM

Cellular Component: adherens junction; apical junction complex; apical part of cell; basolateral plasma membrane; beta-catenin destruction complex; catenin complex; cell cortex; cell junction; cell projection membrane; cell-cell adherens junction; centrosome; cytoplasm; cytosol; dendritic shaft; fascia adherens; flotillin complex; focal adhesion; intercellular junction; lamellipodium; lateral plasma membrane; membrane; microvillus membrane; neuron projection; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; synapse; tight junction; transcription factor complex; Z disc

Molecular Function: alpha-catenin binding; cadherin binding; chromatin binding; DNA binding; double-stranded DNA binding; enzyme binding; estrogen receptor binding; ionotropic glutamate receptor binding; kinase binding; nitric-oxide synthase binding; nuclear hormone receptor binding; protein binding; protein C-terminus binding; protein complex binding; protein heterodimerization activity; protein kinase binding; protein phosphatase binding; SMAD binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: anterior/posterior axis specification; bone resorption; cardiac muscle cell proliferation; cell adhesion; cell differentiation; cell fate determination; cell fate specification; cell maturation; cell proliferation; cell-cell adhesion; cell-matrix adhesion; cellular morphogenesis during differentiation; cellular process; central nervous system vasculogenesis; chromatin-mediated maintenance of transcription; dorsal/ventral axis specification; dorsal/ventral pattern formation; ectoderm development; embryonic axis specification; embryonic digit morphogenesis; embryonic foregut morphogenesis; embryonic forelimb morphogenesis; embryonic heart tube development; embryonic hindlimb morphogenesis; endoderm formation; endodermal cell fate commitment; fallopian tube development; forebrain development; gastrulation with mouth forming second; genitalia morphogenesis; glial cell fate determination; hair cycle process; hair follicle morphogenesis; heart development; hemopoiesis; hindbrain development; in utero embryonic development; kidney development; layer formation in the cerebral cortex; lens morphogenesis in camera-type eye; limb development; lung development; male genitalia development; midbrain development; morphogenesis of embryonic epithelium; negative regulation of cell differentiation; negative regulation of cell proliferation; negative regulation of chondrocyte differentiation; negative regulation of mitotic cell cycle, embryonic; negative regulation of oligodendrocyte differentiation; negative regulation of osteoclast differentiation; negative regulation of protein sumoylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural plate development; neuron differentiation; neuron migration; odontogenesis of dentine-containing teeth; oocyte development; organ development; osteoclast differentiation; pancreas development; patterning of blood vessels; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of endothelial cell differentiation; positive regulation of epithelial cell differentiation; positive regulation of fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of neuroblast proliferation; positive regulation of neuron apoptosis; positive regulation of osteoblast differentiation; positive regulation of skeletal muscle development; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein heterooligomerization; proximal/distal pattern formation; regulation of apoptosis; regulation of cell differentiation; regulation of cell proliferation; regulation of centriole-centriole cohesion; regulation of epithelial cell differentiation; regulation of gene expression; regulation of histone H3-K4 methylation; regulation of myelination; regulation of osteoblast differentiation; regulation of osteoclast differentiation; regulation of smooth muscle cell proliferation; regulation of T cell proliferation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to cytokine stimulus; response to estradiol stimulus; response to estrogen stimulus; Schwann cell proliferation; skeletal development; skin development; smooth muscle cell differentiation; stem cell maintenance; synapse organization and biogenesis; synaptic transmission; synaptic vesicle transport; T cell differentiation; T cell differentiation in the thymus; thymus development; ureteric bud branching; vasculature development; vasculogenesis; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin