Autotaxin / ENPP2 / NPP2 Recombinant Protein | ENPP2 / NPP2 recombinant protein

Recombinant Mouse Autotaxin / ENPP2 / NPP2 Protein (His tag)

Cat. Num. AAA2546858

• Gene Names: Enpp2; ATX; Npps2; Pdnp2; E-NPP 2; lysoPLD; PD-Ialpha
• Purity: > 93 % as determined by SDS-PAGE
• Synonyms: Autotaxin / ENPP2 / NPP2; Recombinant Mouse Autotaxin / ENPP2 / NPP2 Protein (His tag); ATX; Npps2; PD-Ialpha; Pdnp2; ENPP2 / NPP2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 93 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.4
• Sequence Length: 914

• Application Notes: The recombinant mouse ENPP2 comprises 833 amino acids and predicts a molecular mass of 96 kDa. As a result of glycosylation, the apparent molecular mass of mouse ENPP2 is approximately 55-75 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ENPP2 / NPP2 recombinant protein

Background: ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell type s and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.

Description: A DNA sequence encoding the mature form of mouse ENPP2 (Q9R1E6-1) (Ser 49-Ile 862) was fused with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 209862963
• NCBI GeneID: 18606
• NCBI Accession #: NP_001129549.1
• NCBI GenBank Nucleotide #: NM_001136077.3
• UniProt Accession #: Q9R1E6; Q6PDE0; Q99LG9; A8UH85; A8UH93; B2ZP54
• Molecular Weight: 101,680 Da
• NCBI Official Full Name: ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 1
• NCBI Official Synonym Full Names: ectonucleotide pyrophosphatase/phosphodiesterase 2
• NCBI Official Symbol: Enpp2
• NCBI Official Synonym Symbols: ATX; Npps2; Pdnp2; E-NPP 2; lysoPLD; PD-Ialpha
• NCBI Protein Information: ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• UniProt Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• UniProt Gene Name: Enpp2
• UniProt Synonym Gene Names: Npps2; Pdnp2; E-NPP 2; LysoPLD

NCBI Description

This gene encodes a member of the phosphodiesterase and nucleotide pyrophosphatase family of bifunctional enzymes that hydrolize phosphodiester bonds of various nucleotides. The encoded protein undergoes proteolytic processing to generate a mature protein with lysophospholipase D activity, catalyzing the cleavage of the choline group from lysophosphatidylcholine to produce lysophosphatidic acid. This gene is expressed in numerous tissues and participates in neural development, obesity, inflammation and oncogenesis. A complete lack of the encoded protein in mice results in aberrant vascular and neuronal development leading to embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature protein. [provided by RefSeq, Sep 2015]

Uniprot Description

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.39; Lipid Metabolism - ether lipid; Motility/polarity/chemotaxis; Phosphodiesterase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15|15 D1

Cellular Component: cytoplasm; extracellular space; Golgi apparatus; integral to plasma membrane

Molecular Function: alkylglycerophosphoethanolamine phosphodiesterase activity; calcium ion binding; lysophospholipase activity; nucleotide diphosphatase activity; phosphodiesterase I activity; zinc ion binding

Biological Process: negative regulation of cell-matrix adhesion; phospholipid catabolic process; positive regulation of cell proliferation; positive regulation of focal adhesion formation; positive regulation of oligodendrocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell migration