Smad5 recombinant protein

Recombinant Mouse Smad5 Protein

Cat. Num. AAA2546817

• Gene Names: Smad5; Dwf-C; Madh5; MusMLP; AI451355; 1110051M15Rik
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: Smad5; Recombinant Mouse Smad5 Protein; SMAD5; Madh5; Msmad5; Smad5 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH8.0
• Sequence Length: 465

• Application Notes: The recombinant mouse SMAD5 consists of 466 amino acids and predicts a molecular mass of 52.2 KDa. It migrates as an approximately 57 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for Smad5 recombinant protein

Background: SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Description: A DNA sequence encoding the mouse SMAD5 (P97454) (Thr2-Ser465) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 255708405
• NCBI GeneID: 17129
• NCBI Accession #: NP_001157513.1
• NCBI GenBank Nucleotide #: NM_001164041.1
• UniProt Accession #: P97454; P70341; Q810K0
• Molecular Weight: 52,172 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 5
• NCBI Official Synonym Full Names: SMAD family member 5
• NCBI Official Symbol: Smad5
• NCBI Official Synonym Symbols: Dwf-C; Madh5; MusMLP; AI451355; 1110051M15Rik
• NCBI Protein Information: mothers against decapentaplegic homolog 5
• UniProt Protein Name: Mothers against decapentaplegic homolog 5
• Protein Family: SMAD5 antisense gene protein
• UniProt Gene Name: Smad5
• UniProt Synonym Gene Names: Madh5; Msmad5; MAD homolog 5; Mothers against DPP homolog 5; Dwf-C; SMAD 5; Smad5; mSmad5

Uniprot Description

SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 13 B1|13 30.12 cM

Cellular Component: cytoplasm; nucleoplasm; nucleus; protein complex

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: BMP signaling pathway; cardiac muscle contraction; cartilage development; erythrocyte differentiation; germ cell development; Mullerian duct regression; osteoblast fate commitment; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; transforming growth factor beta receptor signaling pathway; ureteric bud development