AKT3 recombinant protein

Recombinant Mouse AKT3 Protein (aa 106-479, His & GST tag)

Cat. Num. AAA2546688

• Gene Names: Akt3; Nmf350; AI851531; D930002M15Rik
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: AKT3; Recombinant Mouse AKT3 Protein (aa 106-479; His & GST tag); AKT3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol
• Sequence Length: 479

• Application Notes: The recombinant mouse AKT3/GST chimera consists of 611 amino acids and has a calculated molecular mass of 71 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for AKT3 recombinant protein

Background: v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.

Description: A DNA sequence encoding the mouse AKT3 (Q9WUA6-1) (Ala106-Glu479) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 190883484
• NCBI GeneID: 23797
• NCBI Accession #: NP_035915.3
• NCBI GenBank Nucleotide #: NM_011785.3
• UniProt Accession #: Q9WUA6
• Molecular Weight: 54,631 Da
• NCBI Official Full Name: RAC-gamma serine/threonine-protein kinase
• NCBI Official Synonym Full Names: thymoma viral proto-oncogene 3
• NCBI Official Symbol: Akt3
• NCBI Official Synonym Symbols: Nmf350; AI851531; D930002M15Rik
• NCBI Protein Information: RAC-gamma serine/threonine-protein kinase
• UniProt Protein Name: RAC-gamma serine/threonine-protein kinase
• Protein Family: RAC-gamma serine/threonine-protein kinase
• UniProt Gene Name: Akt3
• UniProt Synonym Gene Names: PKB gamma

Uniprot Description

Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described.

Protein type: AGC group; AKT family; EC 2.7.11.1; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1|1 H4

Cellular Component: cytoplasm; Golgi apparatus; intracellular; nucleoplasm; plasma membrane

Molecular Function: ATP binding; protein binding; protein kinase activity; protein kinase C binding; protein serine/threonine kinase activity

Biological Process: brain morphogenesis; cellular response to insulin stimulus; homeostasis of number of cells within a tissue; mitochondrial genome maintenance; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of cell size; positive regulation of TOR signaling pathway; protein amino acid phosphorylation; signal transduction