ALCAM / CD166 recombinant protein

Recombinant Human ALCAM / CD166 Protein (His tag)

Cat. Num. AAA2546569

• Gene Names: ALCAM; MEMD; CD166
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: ALCAM / CD166; Recombinant Human ALCAM / CD166 Protein (His tag); CD166; FLJ38514; MEMD; MGC71733; ALCAM; ALCAM / CD166 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 583

• Application Notes: The recombinant human ALCAM consists of 510 amino acids and has a calculated molecular mass of 57.4 kDa. As a result of glycosylation, rh ALCAM migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Trp 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for ALCAM / CD166 recombinant protein

Background: Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

Description: A DNA sequence encoding the extracellular domain (Met 1-Ala 526) of human ALCAM (NP_001618.2) was expressed, with the C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 68163411
• NCBI GeneID: 214
• NCBI Accession #: NP_001618.2
• NCBI GenBank Nucleotide #: NM_001627.3
• UniProt Accession #: Q13740; O60892; Q1HGM8; Q1HGM9; Q6PEY4; Q6ZS95; B2RNS3; B4DTU0
• Molecular Weight: 33,878 Da
• NCBI Official Full Name: CD166 antigen isoform 1
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: ALCAM
• NCBI Official Synonym Symbols: MEMD; CD166
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• UniProt Gene Name: ALCAM

NCBI Description

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Uniprot Description

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.11

Cellular Component: axon; focal adhesion; immunological synapse; integral to plasma membrane; intrinsic to plasma membrane; T cell receptor complex

Molecular Function: protein binding; receptor binding

Biological Process: axon extension involved in axon guidance; cell adhesion; heterophilic cell adhesion; retinal ganglion cell axon guidance; signal transduction