Contactin 1 / CNTN1 Recombinant Protein | CNTN1 recombinant protein

Recombinant Human Contactin 1 / CNTN1 Protein (His tag)

Cat. Num. AAA2546292

• Gene Names: CNTN1; F3; GP135; MYPCN
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Contactin 1 / CNTN1; Recombinant Human Contactin 1 / CNTN1 Protein (His tag); F3; GP135; CNTN1; CNTN1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1018

• Application Notes: The secreted recombinant human CNTN1 comprises 984 amino acids after removal of the signal peptide and has a predicted molecular mass of 110 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCNTN1 is approximately 125 kDa due to glycosylation.
• Predicted N Terminal: Glu 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CNTN1 recombinant protein

Background: Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2 (TAG-1), Contactin-3 (BIG-1), BIG-2, Contactin-5 (NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-1 is a cell surface adhesion molecule that is normally expressed by neurons and oligodendrocytes. Particularly high levels of Contactin-1 are present during brain development. Contactin-1 and Contactin-2 are differentially expressed in a number of neuronal tissues during development, and they interact with several ligands including Nr-CAM, L1, NCAM, neurocan, phosphacan, and tenascin. As a cell adhesion molecule, Contactin-1 plays a role in the formation of axon connections in the developing nervous system. It was demonstrated that Contactin-1 participates in signal pathways via its association with Contactin-associated protein (CNTNAP1), receptor protein tyrosine phosphatase beta (RPTPb) and NOTCH1. Contactin-1 is also involved in paranodal axo-glial junction formation and oligodendrocytes generation. Furthermore, studies indicated that Contactin-1 functions importantly in the invasion and metastasis of lung adenocarcinoma cells. Contactin-1 may also significantly influence the functional expression and distribution of Na+ channels in neurons.

Description: A DNA sequence encoding the amino acid residues (Met 1-993) of human CNTN1 (NP_001834.2) was expressed with the fused C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 28373117
• NCBI GeneID: 1272
• NCBI Accession #: NP_001834.2
• NCBI GenBank Nucleotide #: NM_001843.3
• UniProt Accession #: Q12860; Q12861; Q14030; Q7M4P0; Q8N466; A8K0H9; A8K0Y3
• Molecular Weight: 70,604 Da
• NCBI Official Full Name: contactin-1 isoform 1
• NCBI Official Synonym Full Names: contactin 1
• NCBI Official Symbol: CNTN1
• NCBI Official Synonym Symbols: F3; GP135; MYPCN
• NCBI Protein Information: contactin-1
• UniProt Protein Name: Contactin-1
• Protein Family: Contactin
• UniProt Gene Name: CNTN1

NCBI Description

The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CNTN1: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. Defects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM). CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia. Belongs to the immunoglobulin superfamily. Contactin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: membrane

Disease: Myopathy, Congenital, Compton-north