TRAIL R4 / CD264 / TNFRSF10D recombinant protein

Recombinant Human TRAIL R4 / CD264 / TNFRSF10D Protein (His & Fc tag)

Cat. Num. AAA2546274

• Gene Names: TNFRSF10D; DCR2; CD264; TRUNDD; TRAILR4; TRAIL-R4
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: TRAIL R4 / CD264 / TNFRSF10D; Recombinant Human TRAIL R4 / CD264 / TNFRSF10D Protein (His & Fc tag); TNFRSF10D; CD264; DCR2; TRAILR4; TRUNDD; TRAIL R4 / CD264 / TNFRSF10D recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 386

• Application Notes: The recombinant human TNFRSF10D/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 403 amino acids and predicts a molecular mass of 44.7 kDa. As a result of glycosylation, rhTNFRSF10D/Fc monomer migrates with the molecular mass of approximately 65-80 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 56
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TRAIL R4 / CD264 / TNFRSF10D recombinant protein

Background: Tumor necrosis factor receptor superfamily member 10D (TNFRSF10D), also known as TNF-related apoptosis-inducing ligand receptor 4 (TRAIL R4), CD264, and Decoy receptor 2, is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. TRAIL R4/CD264/TNFRSF10D is widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. TRAIL R4/CD264/TNFRSF10D is also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. The signaling capacity of TRAIL R4 is similar to that of TRAIL R1 and TRAIL R2 with respect to NF-kappaB activation, but differs in its inability to induce apoptosis. TRAIL R4 retains a C-terminal element containing one third of a consensus death domain motif. Transient overexpression of TRAIL R4 in cells normally sensitive to TRAIL-mediated killing confers complete protection, suggesting that one function of TRAIL R4 may be inhibition of TRAIL cytotoxicity.

Description: A DNA sequence encoding the extracellular domain (Met 1-His 211) of humanTNFRSF10D (NP_003831.2) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 22547108
• NCBI GeneID: 8793
• NCBI Accession #: NP_003831.2
• NCBI GenBank Nucleotide #: NM_003840.4
• UniProt Accession #: Q9UBN6; Q9Y6Q4; B2R8W0
• Molecular Weight: 41,823 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 10D
• NCBI Official Synonym Full Names: TNF receptor superfamily member 10d
• NCBI Official Symbol: TNFRSF10D
• NCBI Official Synonym Symbols: DCR2; CD264; TRUNDD; TRAILR4; TRAIL-R4
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 10D
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 10D
• UniProt Gene Name: TNFRSF10D
• UniProt Synonym Gene Names: DCR2; TRAILR4; TRUNDD; DcR2; TRAIL receptor 4; TRAIL-R4

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAIL-R4: Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF- kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway. Widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. Also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; tumor necrosis factor receptor activity

Biological Process: immune response; induction of apoptosis via death domain receptors; inflammatory response; multicellular organismal development; negative regulation of apoptosis; regulation of apoptosis; regulation of cell proliferation; response to lipopolysaccharide; signal transduction