14-3-3 beta / YWHAB recombinant protein

Recombinant Human 14-3-3 beta / YWHAB Protein

Cat. Num. AAA2546049

• Gene Names: YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
• Purity: > 93 % as determined by SDS-PAGE
• Synonyms: 14-3-3 beta / YWHAB; Recombinant Human 14-3-3 beta / YWHAB Protein; GW128; HS1; KCIP-1; YWHAB; 14-3-3 beta / YWHAB recombinant protein

• Host: E Coli
• Purity/Purification: > 93 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.1mM DTT, 10% glycerol, pH 7.5
• Sequence Length: 246

• Application Notes: The recombinant human YWHAB consists of 248 amino acids and has a predicted molecular mass of 28 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for 14-3-3 beta / YWHAB recombinant protein

Background: 14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.

Description: A DNA sequence encoding the human YWHAB (NP_003395.1) (Met 1-Asn 246) was expressed, with two additional amino acids (Gly & Pro) at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4507949
• NCBI GeneID: 7529
• NCBI Accession #: NP_003395.1
• NCBI GenBank Nucleotide #: NM_003404.4
• UniProt Accession #: P31946; A8K9K2; E1P616
• Molecular Weight: 27,850 Da
• NCBI Official Full Name: 14-3-3 protein beta/alpha
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
• NCBI Official Symbol: YWHAB
• NCBI Official Synonym Symbols: HS1; GW128; YWHAA; KCIP-1; HEL-S-1
• NCBI Protein Information: 14-3-3 protein beta/alpha
• UniProt Protein Name: 14-3-3 protein beta/alpha
• UniProt Gene Name: YWHAB
• UniProt Synonym Gene Names: KCIP-1

NCBI Description

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell-cell adherens junction; cytoplasm; cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane; perinuclear region of cytoplasm

Molecular Function: enzyme binding; histone deacetylase binding; phosphoprotein binding; phosphoserine binding; protein binding; protein domain specific binding

Biological Process: cytoplasmic sequestering of protein; MAPKKK cascade; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of protein amino acid dephosphorylation; positive regulation of catalytic activity; regulation of mRNA stability