CD300A recombinant protein

Recombinant Human CD300A Protein (Fc tag)

Gene Names

CD300A; IRC1; IRC2; CLM-8; IRp60; IGSF12; CMRF35H; CMRF-35H; CMRF35-H; CMRF35H9; CMRF35-H9; IRC1/IRC2; CMRF-35-H9

Purity

(97.9+1.1) % as determined by SDS-PAGE

Synonyms

CD300A; Recombinant Human CD300A Protein (Fc tag); CMRF35H; IGSF12; CD300A recombinant protein

Ordering
Cat. Num. AAA2545506 For Research Use Only!

Host

Human Cells

Purity/Purification

(97.9+1.1) % as determined by SDS-PAGE

Form/Format

Lyophilized from sterile PBS, pH 7.4

Sequence Length

186

Application Notes

The recombinant human CD300A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 402 amino acids and has a predicted molecular mass of 44.5 kDa. The apparent molecular mass of the protein is approximately 58-66 kDa in SDS-PAGE under reducing conditions.

Predicted N Terminal

Leu 18

Endotoxin

< 1.0 EU per mug of the protein as determined by the LAL method

Preparation and Storage

Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for CD300A recombinant protein

Background: Mouse CMRF35-like molecule 8, also known as CD300 antigen-like family member A, CMRF35-H9, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CD300a and CMRF35H, is a single-pass type I membrane protein which belongs to the CD300 family. The CD300 family of myeloid immunoglobulin receptors includes activating (CD300b, CD300e) and inhibitory members (CD300a, CD300f), as well as molecules presenting a negative charge within their transmembrane domain (CD300c, CD300d). CD300A / IGSF12 is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.It contains one Ig-like V-type (immunoglobulin-like) domain. CD300A / IGSF12 is an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. CD300c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells. CD300c signaling is partially mediated by a direct association with the immune receptor tyrosine-based activation motif-bearing adaptor FcepsilonRgamma. CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from plasmacytoid dendritic cells (pDCs).

Description: A DNA sequence encoding the human CD300A (Q9UGN4-1) (Met1-Gln178) was expressed with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

NCBI GI #

378786683

NCBI GeneID

11314

NCBI Accession #

NP_001243770.1

NCBI GenBank Nucleotide #

NM_001256841.1

UniProt Accession #

Q9UGN4; O95100; Q9HD97; Q9P0F3; Q9UBK4; Q9UMS9; Q9UMT0; A8MW96

Molecular Weight

16,517 Da

NCBI Official Full Name

CMRF35-like molecule 8 isoform 2

NCBI Official Synonym Full Names

CD300a molecule

NCBI Official Symbol

CD300A

NCBI Official Synonym Symbols

IRC1; IRC2; CLM-8; IRp60; IGSF12; CMRF35H; CMRF-35H; CMRF35-H; CMRF35H9; CMRF35-H9; IRC1/IRC2; CMRF-35-H9

NCBI Protein Information

CMRF35-like molecule 8

UniProt Protein Name

CMRF35-like molecule 8

Protein Family

CMRF35-like molecule

UniProt Gene Name

CD300A

UniProt Synonym Gene Names

CMRF35H; IGSF12; CLM-8; CMRF35-H9; IgSF12; IRp60

NCBI Description

This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

MAIR-I: Inhibitory receptor which may contribute to the down- regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Belongs to the CD300 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: membrane; plasma membrane

Molecular Function: phosphatidylethanolamine binding; phosphatidylserine binding; protein binding

Biological Process: negative regulation of B cell proliferation; negative regulation of B cell receptor signaling pathway; negative regulation of fibroblast proliferation; negative regulation of MAP kinase activity; negative regulation of mast cell activation during immune response; negative regulation of mast cell degranulation; negative regulation of NK T cell activation; negative regulation of phagocytosis, engulfment; positive regulation of phosphoprotein phosphatase activity; regulation of immune response; regulation of T cell receptor signaling pathway

Research Articles on CD300A

1. High CD300A expression is associated with diffuse large B-cell lymphoma.

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Product Notes

The CD300A cd300a (Catalog #AAA2545506) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human CD300A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 402 amino acids and has a predicted molecular mass of 44.5 kDa. The apparent molecular mass of the protein is approximately 58-66 kDa in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the CD300A cd300a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD300A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.