SerpinB9 / CAP-3 Recombinant Protein | CAP-3 recombinant protein

Recombinant Human SerpinB9 / CAP-3 Protein (His tag)

Cat. Num. AAA2545448

• Gene Names: SERPINB9; PI9; CAP3; PI-9; CAP-3
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: SerpinB9 / CAP-3; Recombinant Human SerpinB9 / CAP-3 Protein (His tag); CAP-3; CAP3; PI9; CAP-3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4
• Sequence Length: 376

• Application Notes: The recombinant human SERPINB9 consists of 394 amino acids and predicts a molecular mass of 44.5 kDa. It migrates as an approximately 43 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CAP-3 recombinant protein

Background: SerpinB9, also known as Cytoplasmic antiproteinase 3, CAP-3, Peptidase inhibitor 9, SERPINB9 and PI-9, is a cytoplasm protein which belongs to the serpin family and Ov-serpin subfamily. Serpin-B9 (CAP-3 / PI-9) is the only known human intracellular inhibitor of granzyme B (GrB), the effector molecule in immunity against cytomegalovirus (CMV) and in renal allograft rejection. Serpin-B9 and SPI-6 expression in immune-privileged cells, APCs, and CTLs protects these cells against the actions of granzyme B, and when expressed in tumor cells or virally infected hepatocytes, confers resistance to killing by CTL and NK cells. Expression of increasing levels of Serpin-B9 (CAP-3 / PI-9) in target cells may progressively inhibit immune surveillance by blocking NK and CTL-induced cytotoxicity through the perforin / granzyme pathway and then through the Fas / FasL pathway. Serpin-B9 (CAP-3 / PI-9) is selectively up-regulated in hepatocytes in response to infiltration of the liver by NK cells that express perforin and enzymatically active granzyme B. Upregulated expression of Serpin-B9 (CAP-3 / PI-9) in NSCLC cells may serve to protect them from apoptosis induced by GrB.

Description: A DNA sequence encoding the human SERPINB9 (P50453) (Glu 2-Pro 376) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4758906
• NCBI GeneID: 5272
• NCBI Accession #: NP_004146.1
• NCBI GenBank Nucleotide #: NM_004155.5
• UniProt Accession #: P50453; Q5TD03; B2RBW3
• Molecular Weight: 42,404 Da
• NCBI Official Full Name: serpin B9
• NCBI Official Synonym Full Names: serpin family B member 9
• NCBI Official Symbol: SERPINB9
• NCBI Official Synonym Symbols: PI9; CAP3; PI-9; CAP-3
• NCBI Protein Information: serpin B9
• UniProt Protein Name: Serpin B9
• UniProt Gene Name: SERPINB9
• UniProt Synonym Gene Names: PI9; CAP-3; CAP3; PI-9

NCBI Description

This gene encodes a member of the serine protease inhibitor family which are also known as serpins. The encoded protein belongs to a subfamily of intracellular serpins. This protein inhibits the activity of the effector molecule granzyme B. Overexpression of this protein may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

SERPINB9: Granzyme B inhibitor. Belongs to the serpin family. Ov-serpin subfamily.

Chromosomal Location of Human Ortholog: 6p25.2

Cellular Component: cytoplasm; cytosol; extracellular space; intracellular; membrane; nucleus

Molecular Function: caspase inhibitor activity; protease binding; protein binding; serine-type endopeptidase inhibitor activity

Biological Process: immune response; mast cell mediated immunity; negative regulation by symbiont of host apoptosis; negative regulation of apoptosis; protection from natural killer cell mediated cytotoxicity; response to bacterium