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SDS-PAGE

SMPD1 / ASM recombinant protein

Recombinant Human SMPD1 / ASM Protein (His tag)

Gene Names
SMPD1; ASM; NPD; ASMASE
Purity
> 94 % as determined by SDS-PAGE
Synonyms
SMPD1 / ASM; Recombinant Human SMPD1 / ASM Protein (His tag); ASM; NPD; SMPD1; SMPD1 / ASM recombinant protein
Ordering
For Research Use Only!
Host
Baculovirus-Insect Cells
Purity/Purification
> 94 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.1% OGP, 10% glycerol
Sequence Length
631
Application Notes
The secreted recombinant human SMPD1 consists of 518 amino acids and predicts a molecular mass of 65 kDa as estimated by SDS-PAGE under reducing conditions.
Predicted N Terminal
His 62
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

SDS-PAGE
Related Product Information for SMPD1 / ASM recombinant protein
Background: Sphingomyelin phosphodiesterase 1 (SMPD1), also known as ASM (acid sphingomyelinase), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86% aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.

Description: A DNA sequence encoding the full length of human SMPD1 isoform 1 (NP_000534.3) (Met 1-Cys 631) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,624 Da
NCBI Official Full Name
sphingomyelin phosphodiesterase isoform 1
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 1
NCBI Official Symbol
SMPD1
NCBI Official Synonym Symbols
ASM; NPD; ASMASE
NCBI Protein Information
sphingomyelin phosphodiesterase
UniProt Protein Name
Sphingomyelin phosphodiesterase
UniProt Gene Name
SMPD1
UniProt Synonym Gene Names
ASM; aSMase

NCBI Description

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

Uniprot Description

SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.12; Lipid Metabolism - sphingolipid; Phosphodiesterase

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: endosome; extracellular space; lysosomal lumen; lysosome; plasma membrane

Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; zinc ion binding

Biological Process: ceramide biosynthetic process; glycosphingolipid metabolic process; negative regulation of MAP kinase activity; nervous system development; positive regulation of protein amino acid dephosphorylation; signal transduction; sphingomyelin catabolic process; sphingomyelin metabolic process

Disease: Niemann-pick Disease, Type A; Niemann-pick Disease, Type B

Research Articles on SMPD1 / ASM

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Product Notes

The SMPD1 / ASM smpd1 (Catalog #AAA2545072) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant human SMPD1 consists of 518 amino acids and predicts a molecular mass of 65 kDa as estimated by SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the SMPD1 / ASM smpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMPD1 / ASM, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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