FES Kinase / Feline sarcoma oncogene Recombinant Protein | FES recombinant protein

Recombinant Human FES Kinase / Feline sarcoma oncogene Protein (His & GST tag)

Cat. Num. AAA2545039

• Gene Names: FES; FPS
• Purity: > 75 % as determined by SDS-PAGE
• Synonyms: FES Kinase / Feline sarcoma oncogene; Recombinant Human FES Kinase / Feline sarcoma oncogene Protein (His & GST tag); FPS; FES; FES recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 75 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
• Sequence Length: 764

• Application Notes: The recombinant human FES/GST chimera consists of 1060 amino acids and has a calculated molecular mass of 121 kDa. It migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for FES recombinant protein

Background: Proto-oncogene tyrosine-protein kinase Fes/Fps, also known as Proto-oncogene c-Fes, Proto-oncogene c-Fps, Feline sarcoma oncogene, FES and FPS, is a protein which contains one FCH domain, one protein kinase domain and one SH2 domain. FES is a non-receptor protein tyrosine kinase expressed in hematopoietic progenitors and differentiated myeloid cells. FES is observed in the nuclear, granular and plasma membrane fractions of primary human neutrophils and the myeloid leukemia cell line, HL-60. The nuclear localization is confirmed by immunocytochemistry of neutrophils. FES has been implicated in granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) and erythropoietin signal transduction. FES has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. FES is also involved in normal hematopoiesis. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia.

Description: A DNA sequence encoding the human FES (P07332-1) (Met 1-Arg 822) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 219842230
• NCBI GeneID: 2242
• NCBI Accession #: NP_001137255.1
• NCBI GenBank Nucleotide #: NM_001143783.1
• UniProt Accession #: P07332; Q2VXS7; Q2VXS8; Q2VXT0; Q6GTU5; B2R6E6; B4DUD0; E9PC94; E9PC95
• Molecular Weight: 85,466 Da
• NCBI Official Full Name: tyrosine-protein kinase Fes/Fps isoform 2
• NCBI Official Synonym Full Names: FES proto-oncogene, tyrosine kinase
• NCBI Official Symbol: FES
• NCBI Official Synonym Symbols: FPS
• NCBI Protein Information: tyrosine-protein kinase Fes/Fps
• UniProt Protein Name: Tyrosine-protein kinase Fes/Fps
• Protein Family: (-)-endo-fenchol synthase
• UniProt Gene Name: FES
• UniProt Synonym Gene Names: FPS

NCBI Description

This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms.[provided by RefSeq, Jan 2009]

Uniprot Description

Fes: a non-receptor tyrosine kinase of the Fer family. Appears to be involved in normal hematopoiesis as well as the development of acute promyelocytic leukemia. Contains one SH2 and one FCH domain. Four LOF point mutations seen in colorectal cancer. Orthologous to v-fes from feline leukemia virus and v-fps from avian transforming virus. Mutant forms are angiogenic. Promotes survival during differentiation, and may act both to promote and inhibit tumors. May be disrupted in the t(15q+;17q-) found in acute promyelocytic leukemia, but the breakpoint does not occur within the gene.

Protein type: EC 2.7.10.2; Fer family; Kinase, protein; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Tumor suppressor

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; extrinsic to internal side of plasma membrane; focal adhesion; microtubule cytoskeleton

Molecular Function: non-membrane spanning protein tyrosine kinase activity; phosphoinositide binding; protein binding; protein-tyrosine kinase activity

Biological Process: cell proliferation; chemotaxis; epidermal growth factor receptor signaling pathway; innate immune response; multicellular organismal development; peptidyl-tyrosine phosphorylation; positive regulation of microtubule polymerization; positive regulation of myeloid cell differentiation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell adhesion; regulation of cell differentiation; regulation of cell proliferation; regulation of cell shape; regulation of mast cell degranulation