AK4 / Adenylate Kinase 4 / AK3L1 Recombinant Protein | AK4 recombinant protein

Recombinant Human AK4 / Adenylate Kinase 4 / AK3L1 Protein (His & GST tag)

Cat. Num. AAA2545037

• Gene Names: AK4; AK3; AK 4; AK3L1; AK3L2
• Purity: > 94 % as determined by SDS-PAGE
• Synonyms: AK4 / Adenylate Kinase 4 / AK3L1; Recombinant Human AK4 / Adenylate Kinase 4 / AK3L1 Protein (His & GST tag); RP4-686B20.1; AK3; AK3L1; AK3L2; MGC166959; AK4 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 94 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0
• Sequence Length: 223

• Application Notes: The recombinant human AK4/GST chimera consists of 460 amino acids and has a calculated molecular mass of 53 kDa. It migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for AK4 recombinant protein

Background: Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 (AK4 / AK3L1) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

Description: A DNA sequence encoding the human AK4 (P27144-1) (Ala 2-Tyr 223) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 53832003
• NCBI GeneID: 205
• NCBI Accession #: NP_001005353.1
• NCBI GenBank Nucleotide #: NM_001005353.2
• UniProt Accession #: P27144; Q6IBH4; Q6NXQ5; Q8IUU9; B2R927; D3DQ62
• Molecular Weight: 25,268 Da
• NCBI Official Full Name: adenylate kinase 4, mitochondrial isoform a
• NCBI Official Synonym Full Names: adenylate kinase 4
• NCBI Official Symbol: AK4
• NCBI Official Synonym Symbols: AK3; AK 4; AK3L1; AK3L2
• NCBI Protein Information: adenylate kinase 4, mitochondrial
• UniProt Protein Name: Adenylate kinase 4, mitochondrial
• UniProt Gene Name: AK4
• UniProt Synonym Gene Names: AK 4

NCBI Description

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

AK3L1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. May also be active with GTP. Belongs to the adenylate kinase family.

Protein type: EC 2.7.4.10; EC 2.7.4.6; Kinase, other; Mitochondrial; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: adenylate kinase activity; nucleoside diphosphate kinase activity; nucleoside phosphate kinase activity; nucleoside triphosphate adenylate kinase activity

Biological Process: AMP metabolic process; ATP metabolic process; GTP metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleoside diphosphate phosphorylation; nucleoside triphosphate biosynthetic process