ERK3 / MAPK12 / P38-gamma recombinant protein

Recombinant Human ERK3 / MAPK12 / P38-gamma Protein (His & GST tag)

Cat. Num. AAA2545019

• Gene Names: MAPK12; ERK3; ERK6; ERK-6; SAPK3; PRKM12; SAPK-3; MAPK 12; P38GAMMA
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: ERK3 / MAPK12 / P38-gamma; Recombinant Human ERK3 / MAPK12 / P38-gamma Protein (His & GST tag); MAPK12; ERK6; SAPK3; ERK3 / MAPK12 / P38-gamma recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
• Sequence Length: 357

• Application Notes: The recombinant human MAPK12 /GST chimera consists of 604 amino acids and has a calculated molecular mass of 69.8 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ERK3 / MAPK12 / P38-gamma recombinant protein

Background: ERK3, also known as MAPK12 and p38-gamma, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. ERK3 is highly expressed in skeletal muscle and heart. ERK3 is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.

Description: A DNA sequence encoding the human MAPK12 (P53778) (Met1-Leu367) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 735997389
• NCBI GeneID: 6300
• NCBI Accession #: NP_001290181.1
• NCBI GenBank Nucleotide #: NM_001303252.2
• UniProt Accession #: P53778; Q14260; Q6IC53; Q99588; Q99672
• Molecular Weight: 40,808 Da
• NCBI Official Full Name: mitogen-activated protein kinase 12 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 12
• NCBI Official Symbol: MAPK12
• NCBI Official Synonym Symbols: ERK3; ERK6; ERK-6; SAPK3; PRKM12; SAPK-3; MAPK 12; P38GAMMA
• NCBI Protein Information: mitogen-activated protein kinase 12
• UniProt Protein Name: Mitogen-activated protein kinase 12
• UniProt Gene Name: MAPK12
• UniProt Synonym Gene Names: ERK6; SAPK3; MAP kinase 12; MAPK 12; ERK-6; MAP kinase p38 gamma

NCBI Description

Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes; and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.