Human Mitogen Activated Protein Kinase 12 (MAPK12) ELISA Kit | MAPK12 elisa kit

Human Mitogen Activated Protein Kinase 12 (MAPK12) ELISA Kit

Cat. Num. AAA454622

• Gene Names: MAPK12; ERK3; ERK6; ERK-6; SAPK3; PRKM12; SAPK-3; MAPK 12; P38GAMMA
• Reactivity: Human
• Synonyms: Mitogen Activated Protein Kinase 12 (MAPK12); Human Mitogen Activated Protein Kinase 12 (MAPK12) ELISA Kit; MAPK12 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MAPK12. No significant cross-reactivity or interference between MAPK12 and analogues was observed.
• Sequence Length: 357

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.072ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MAPK12 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MAPK12 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAPK12 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MAPK12 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAPK12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MAPK12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAPK12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MAPK12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 735997389
• NCBI GeneID: 6300
• NCBI Accession #: NP_001290181.1
• NCBI GenBank Nucleotide #: NM_001303252.2
• UniProt Accession #: P53778; Q14260; Q6IC53; Q99588; Q99672
• Molecular Weight: 40,808 Da
• NCBI Official Full Name: mitogen-activated protein kinase 12 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 12
• NCBI Official Symbol: MAPK12
• NCBI Official Synonym Symbols: ERK3; ERK6; ERK-6; SAPK3; PRKM12; SAPK-3; MAPK 12; P38GAMMA
• NCBI Protein Information: mitogen-activated protein kinase 12
• UniProt Protein Name: Mitogen-activated protein kinase 12
• UniProt Gene Name: MAPK12
• UniProt Synonym Gene Names: ERK6; SAPK3; MAP kinase 12; MAPK 12; ERK-6; MAP kinase p38 gamma

NCBI Description

Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes; and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.