Rabbit anti-Human PARK7  Monoclonal Antibody | anti-PARK7  antibody

Rabbit anti Human PARK7  Monoclonal Antibody

Cat. Num. AAA2544369

• Gene Names: PARK7; DJ1; DJ-1; HEL-S-67p
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: PARK7 ; Monoclonal Antibody; Rabbit anti Human PARK7 Monoclonal Antibody; anti-PARK7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 189

• Applicable Applications for anti-PARK7 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 0.5-1 mug/mL; Flow Cytometry: 0.5-2 mug/Test; ICC/IF: 10-25 mug/mL; IP: 2-4 mug/mg of lysate
• Immunogen: Recombinant Human PARK7/DJ-1 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-PARK7  antibody

Parkinson's disease locus DJ-1 (PARK7) is a differentially expressed transcript. DJ-1 plays a physiologic role in protection of erythroid cells from oxidant damage, a function unmasked in the context of oxidative stress. PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson's disease (PD). DJ-1/p53 interactions contribute to apoptosis resistance in clonal myeloid cells and may serve as a prognostic marker in patients with myelodysplastic syndromes (MDS). DJ-1 regulates redox signaling kinase pathways and acts as a transcriptional regulator of antioxidative gene batteries. Therefore, DJ-1 is an important redox-reactive signaling intermediate controlling oxidative stress after ischemia, upon neuroinflammation, and during age-related neurodegenerative processes. Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson's disease and acute damage such as stroke.

NCBI and Uniprot Product Information

• NCBI GI #: 183227678
• NCBI GeneID: 11315
• NCBI GenBank Nucleotide #: NM_001123377.1
• Molecular Weight: 19,891 Da
• NCBI Official Full Name: protein deglycase DJ-1
• NCBI Official Synonym Full Names: Parkinsonism associated deglycase
• NCBI Official Symbol: PARK7
• NCBI Official Synonym Symbols: DJ1; DJ-1; HEL-S-67p
• NCBI Protein Information: protein deglycase DJ-1
• UniProt Protein Name: Protein deglycase DJ-1
• UniProt Gene Name: PARK7
• UniProt Synonym Gene Names: DJ-1
• UniProt Entry Name: PARK7_HUMAN

NCBI Description

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: EC 3.4.-.-; Nuclear receptor co-regulator; Transcription regulation; Tumor suppressor

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: axon; cell-cell adherens junction; chromatin; cytoplasm; cytosol; mitochondrial respiratory chain complex I; mitochondrion; nucleus; perinuclear region of cytoplasm; PML body

Molecular Function: androgen receptor binding; copper ion binding; cytokine binding; double-stranded DNA binding; enzyme binding; identical protein binding; kinase binding; mercury ion binding; mRNA binding; oxidoreductase activity, acting on peroxide as acceptor; peptidase activity; protein binding; protein homodimerization activity; receptor binding; single-stranded DNA binding; superoxide dismutase copper chaperone activity; transcription coactivator activity; transcription factor binding

Biological Process: activation of protein kinase B; detoxification of copper ion; detoxification of mercury ion; glycolate biosynthetic process; hydrogen peroxide metabolic process; lactate biosynthetic process; methylglyoxal metabolic process; mitochondrion organization and biogenesis; negative regulation of apoptosis; negative regulation of neuron apoptosis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein amino acid phosphorylation; negative regulation of protein binding; negative regulation of protein export from nucleus; negative regulation of protein kinase activity; negative regulation of protein sumoylation; negative regulation of protein ubiquitination; negative regulation of ubiquitin-protein ligase activity; positive regulation of interleukin-8 production; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; protein deglycosylation; protein stabilization; Ras protein signal transduction; regulation of inflammatory response; regulation of mitochondrial membrane potential; regulation of neuron apoptosis; regulation of TRAIL receptor biosynthetic process

Disease: Parkinson Disease 7, Autosomal Recessive Early-onset