Rabbit anti-Human CEACAM8  Monoclonal Antibody | anti-CEACAM8  antibody

Rabbit anti Human CEACAM8  Monoclonal Antibody

Cat. Num. AAA2544281

• Gene Names: CEACAM8; CD67; CGM6; CD66b; NCA-95
• Reactivity: Human
• Applications: ELISA
• Synonyms: CEACAM8 ; Monoclonal Antibody; Rabbit anti Human CEACAM8 Monoclonal Antibody; anti-CEACAM8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 349

• Applicable Applications for anti-CEACAM8 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human CEACAM8. The detection limit for Human CEACAM8 is approximately 0. 00245 ng/well.
• Immunogen: Recombinant Human CEACAM8/CD66b protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CEACAM8  antibody

CEACAM8, also known as CD66b or NCA-95, is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic antigen family. There are four members in this family: CD66a, CD66b, CD66c, and CD66d. Members of CEACAM family are widely expressed especially on human neutrophils, and, depending on the tissue, capable of regulating diverse functions including tumor promotion, tumor suppression, angiogenesis, and neutrophil activation. Abnormal overexpression and downregulation of some CEACAMs have been described in tumor cells. Monoclonal antibodies grouped in the CD66 cluster recognize CEACAM members. Ectopic CD66 expression is commonly detected in B-cell lineage acute lymphoblastic leukemia (ALL). CEACAM8 (CD66b) is also an activation marker for human granulocytes. However, its biological functions are largely unknown in eosinophils. It has been reported that CD66b is highly expressed on the surface of human peripheral blood eosinophils isolated from healthy individuals. Engagement of CD66b by mAb or a natural ligand, galectin-3, activated a Src kinase family molecule, hemopoietic cell kinase (Hck), and induced cellular adhesion, superoxide production, and degranulation of eosinophils. CD66b molecules were localized in lipid rafts, and disruption of lipid rafts or removal of the GPI anchor inhibited the adhesion and activation of eosinophils. Importantly, CD66b was constitutively and physically associated with a beta2 integrin, CD11b, and cross-linking of CD66b induced a striking clustering of CD11b molecules. Thus, CD66b molecules are involved in regulating adhesion and activation of eosinophils, possibly through their localization in lipid rafts and interaction with other cell surface molecules, such as CD11b. Binding of exogenous or endogenous carbohydrate ligands (s) to CD66b may be important in the release of proinflammatory mediators by human eosinophils.

NCBI and Uniprot Product Information

• NCBI GI #: 21314600
• NCBI GeneID: 1088
• NCBI GenBank Nucleotide #: NM_001816.3
• Molecular Weight: 38,154 Da
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 8
• NCBI Official Synonym Full Names: carcinoembryonic antigen related cell adhesion molecule 8
• NCBI Official Symbol: CEACAM8
• NCBI Official Synonym Symbols: CD67; CGM6; CD66b; NCA-95
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 8
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 8
• UniProt Gene Name: CEACAM8
• UniProt Synonym Gene Names: CGM6
• UniProt Entry Name: CEAM8_HUMAN

Uniprot Description

CEACAM8: Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Immunoglobulin superfamily; Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: immune response; leukocyte migration