Mouse anti-Human Ephrin-A1/EFNA1 Monoclonal Antibody | anti-EFNA1 antibody

Mouse anti Human Ephrin-A1/EFNA1 Monoclonal Antibody

Cat. Num. AAA2543495

• Gene Names: Efna1; B61; Efl1; Epl1; Eplg1; Lerk1; AI325262
• Reactivity: Human
• Applications: ELISA
• Synonyms: Ephrin-A1/EFNA1; Monoclonal Antibody; Mouse anti Human Ephrin-A1/EFNA1 Monoclonal Antibody; anti-EFNA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Sequence Length: 205

• Applicable Applications for anti-EFNA1 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.5-1 mug/mL. This antibody can be used at 0.5-1 mug/mL with the appropriate secondary reagents to detect Human EFNA1. The detection limit for Human EFNA1 is 0.078 ng/well.
• Immunogen: Recombinant Human EFNA1 protein
• Buffer: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EphrinA1 (rh EphrinA1; NP_004419.2; Met 1-Ser 182). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 247269690
• NCBI GeneID: 13636
• NCBI GenBank Nucleotide #: NM_010107.4
• Molecular Weight: 23,802 Da
• NCBI Official Full Name: ephrin-A1 isoform 1
• NCBI Official Synonym Full Names: ephrin A1
• NCBI Official Symbol: Efna1
• NCBI Official Synonym Symbols: B61; Efl1; Epl1; Eplg1; Lerk1; AI325262
• NCBI Protein Information: ephrin-A1
• UniProt Protein Name: Ephrin-A1
• Protein Family: Ephrin
• UniProt Gene Name: Efna1
• UniProt Synonym Gene Names: Epgl1; Epl1; Lerk1; LERK-1
• UniProt Entry Name: EFNA1_MOUSE

Uniprot Description

EFNA1: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis. Belongs to the ephrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Ligand, receptor tyrosine kinase; Membrane protein, GPI anchor; Tumor suppressor

Cellular Component: anchored to plasma membrane

Molecular Function: ephrin receptor binding

Biological Process: activation of MAPK activity; axon guidance; cell migration; ephrin receptor signaling pathway; MAPKKK cascade; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; notochord formation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of angiogenesis; regulation of axonogenesis; regulation of blood vessel endothelial cell migration; regulation of cell adhesion mediated by integrin; regulation of peptidyl-tyrosine phosphorylation