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Western Blot (WB) (Western Blot analysis of Human brain tissue using NENF Monoclonal Antibody at dilution of 1:1000)

Mouse anti-Human, Mouse NENF Monoclonal Antibody | anti-NENF antibody

NENF Monoclonal Antibody

Gene Names
NENF; CIR2; SPUF; SCIRP10
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Protein A purification
Synonyms
NENF; Monoclonal Antibody; NENF Monoclonal Antibody; Cell growth inhibiting protein 47; Cell immortalization-related protein 2; CIR2; Nenf; Neudesin; Neuron derived neurotrophic factor; Neuron-derived neurotrophic factor; SCIRP10; SCIRP10 related protein; Secreted protein of unknown function; Spinal cord injury related protein 10; SPUF; SPUF protein; anti-NENF antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6540
Purity/Purification
Protein A purification
Concentration
1mg/mL (varies by lot)
Sequence Length
172
Applicable Applications for anti-NENF antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
Immunogen
Fusion protein of NENF
Buffer
PBS with 0.1% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Human brain tissue using NENF Monoclonal Antibody at dilution of 1:1000)

Western Blot (WB) (Western Blot analysis of Human brain tissue using NENF Monoclonal Antibody at dilution of 1:1000)
Related Product Information for anti-NENF antibody
This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants.NENF (Neudesin Neurotrophic Factor) is a Protein Coding gene. GO annotations related to this gene include growth factor activity and heme binding. An important paralog of this gene is CYB5D2.
Product Categories/Family for anti-NENF antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 19kDa
Observed: 18kDa
NCBI Official Full Name
neudesin
NCBI Official Synonym Full Names
neudesin neurotrophic factor
NCBI Official Symbol
NENF
NCBI Official Synonym Symbols
CIR2; SPUF; SCIRP10
NCBI Protein Information
neudesin
UniProt Protein Name
Neudesin
Protein Family
UniProt Gene Name
NENF
UniProt Synonym Gene Names
SPUF protein

NCBI Description

This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2009]

Uniprot Description

Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (). Plays a role in the human tumorigenesis (PubMed:22748190).

Research Articles on NENF

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Product Notes

The NENF nenf (Catalog #AAA2542211) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NENF Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NENF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:50-1:500. Researchers should empirically determine the suitability of the NENF nenf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NENF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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