Western Blot (WB)
(Western Blot analysis of Human brain lysate using MGEA5 Monoclonal Antibody at dilution of 1:600)
Mouse anti-Human MGEA5 Monoclonal Antibody | anti-MGEA5 antibody
MGEA5 Monoclonal Antibody
Gene Names
OGA; MEA5; MGEA5; NCOAT
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Protein G purification
Synonyms
MGEA5; Monoclonal Antibody; MGEA5 Monoclonal Antibody; Beta-hexosaminidase; Beta-N-acetylglucosaminidase; Beta-N-acetylhexosaminidase; Bifunctional protein NCOAT; HEXC; Hyaluronidase in meningioma; KIAA0679; MEA5; Meningioma expressed antigen 5; Meningioma-expressed antigen 5; MGEA 5; Mgea5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; Nuclear cytoplasmic O GlcNAcase and acetyltransferase; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; OGA; Protein O-GlcNAcase; anti-MGEA5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7012
Purity/Purification
Protein G purification
Concentration
1mg/mL (varies by lot)
Sequence Length
863
Applicable Applications for anti-MGEA5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
IHC: 1:20-1:200
IF: 1:20-1:200
Immunogen
Fusion protein of MGEA5
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of Human brain lysate using MGEA5 Monoclonal Antibody at dilution of 1:600)
Western Blot (WB)
(Western Blot analysis of Human brain lysate using MGEA5 Monoclonal Antibody at dilution of 1:600)
Related Product Information for anti-MGEA5 antibody
Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present mouse monopolyclonal antibody MGEA5 Antibody, which is consistent with results in a related reference (PubMed:11341771).
Product Categories/Family for anti-MGEA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 103kDa
Observed: 75kDa; 130kDa
Observed: 75kDa; 130kDa
NCBI Official Full Name
protein O-GlcNAcase isoform b
NCBI Official Synonym Full Names
O-GlcNAcase
NCBI Official Symbol
OGA
NCBI Official Synonym Symbols
MEA5; MGEA5; NCOAT
NCBI Protein Information
protein O-GlcNAcase
UniProt Protein Name
Protein O-GlcNAcase
Protein Family
UniProt Gene Name
MGEA5
UniProt Synonym Gene Names
HEXC; KIAA0679; MEA5; NCOAT
NCBI Description
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
Uniprot Description
Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
Research Articles on MGEA5
Similar Products
Product Notes
The MGEA5 mgea5 (Catalog #AAA2541983) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MGEA5 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGEA5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:20-1:200. Researchers should empirically determine the suitability of the MGEA5 mgea5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGEA5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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