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Western Blot (WB) (Western Blot analysis of HeLa cells using CYB5R3 Monoclonal Antibody at dilution of 1:60000)

Mouse anti-Human CYB5R3 Monoclonal Antibody | anti-CYB5R3 antibody

CYB5R3 Monoclonal Antibody

Gene Names
CYB5R3; B5R; DIA1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Protein A purification
Synonyms
CYB5R3; Monoclonal Antibody; CYB5R3 Monoclonal Antibody; B5R; Cyb5r3; Cytochrome b5 reductase 3; Cytochrome b5 reductase; DIA1; Diaphorase 1; Diaphorase-1; NADH cytochrome b5 reductase 3; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form; NB5R3; OTTHUMP00000028761; OTTHUMP00000198435; OTTHUMP00000198574; OTTHUMP00000198662; OTTHUMP00000198665; anti-CYB5R3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
227
Purity/Purification
Protein A purification
Concentration
1mg/mL (varies by lot)
Sequence Length
301
Applicable Applications for anti-CYB5R3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:5000-1:50000
IHC: 1:20-1:200
IF: 1:50-1:500
Immunogen
Fusion protein of CYB5R3
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of HeLa cells using CYB5R3 Monoclonal Antibody at dilution of 1:60000)

Western Blot (WB) (Western Blot analysis of HeLa cells using CYB5R3 Monoclonal Antibody at dilution of 1:60000)
Related Product Information for anti-CYB5R3 antibody
CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.
Product Categories/Family for anti-CYB5R3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 33kDa
Observed: 33kDa
NCBI Official Full Name
NADH-cytochrome b5 reductase 3 isoform 1
NCBI Official Synonym Full Names
cytochrome b5 reductase 3
NCBI Official Symbol
CYB5R3
NCBI Official Synonym Symbols
B5R; DIA1
NCBI Protein Information
NADH-cytochrome b5 reductase 3
UniProt Protein Name
NADH-cytochrome b5 reductase 3
UniProt Gene Name
CYB5R3
UniProt Synonym Gene Names
DIA1; B5R; Cytochrome b5 reductase

NCBI Description

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq, Jan 2010]

Uniprot Description

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

Research Articles on CYB5R3

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Product Notes

The CYB5R3 cyb5r3 (Catalog #AAA2541889) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CYB5R3 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYB5R3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:5000-1:50000 IHC: 1:20-1:200 IF: 1:50-1:500. Researchers should empirically determine the suitability of the CYB5R3 cyb5r3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYB5R3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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