Typical Testing Data/Standard Curve (for reference only)
Rat Serotonin Transporter ELISA Kit | SERT elisa kit
Rat SERT (Serotonin Transporter) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SERT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SERT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SERT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat SERT. You can calculate the concentration of Rat SERT in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma. [provided by RefSeq, Jul 2008]
Uniprot Description
SERT: Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: cytosol; endomembrane system; endosome membrane; integral to plasma membrane; lipid raft; neuron projection; plasma membrane
Molecular Function: actin filament binding; cocaine binding; monoamine transmembrane transporter activity; myosin binding; nitric-oxide synthase binding; protein binding; protein homodimerization activity; Rab GTPase binding; serotonin transmembrane transporter activity; serotonin:sodium symporter activity; syntaxin-1 binding
Biological Process: brain morphogenesis; circadian rhythm; memory; monoamine transport; negative regulation of granule cell precursor proliferation; negative regulation of neuron differentiation; negative regulation of organ growth; negative regulation of synaptic transmission, dopaminergic; positive regulation of cell cycle; protein homooligomerization; protein oligomerization; response to drug; response to estradiol stimulus; response to hypoxia; response to nutrient; response to toxin; serotonin transport; serotonin uptake; social behavior; sperm ejaculation; synaptic transmission; thalamus development; transmembrane transport; vasoconstriction
Disease: Anxiety; Obsessive-compulsive Disorder
Research Articles on SERT
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Product Notes
The Rat SERT slc6a4 (Catalog #AAA2533556) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533556 ELISA Kit recognizes Rat SERT. It is sometimes possible for the material contained within the vial of "Serotonin Transporter, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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