Typical Testing Data/Standard Curve (for reference only)
Mouse HNF4a (Hepatocyte Nuclear Factor 4 Alpha) CLIA Kit | HNF4a clia kit
Mouse HNF4a (Hepatocyte Nuclear Factor 4 Alpha) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to HNF4a. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HNF4a and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HNF4a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of HNF4a. You can calculate the concentration of HNF4a in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
steriod horomone receptor; involved in promoting transcription [RGD, Feb 2006]
Uniprot Description
HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described.
Protein type: Transcription factor; DNA-binding; Nuclear receptor
Cellular Component: cytoplasm; nucleus; transcription factor complex
Molecular Function: acyl-CoA binding; DNA binding; fatty acid binding; ligand-dependent nuclear receptor activity; palmitoyl-CoA hydrolase activity; protein binding; protein homodimerization activity; receptor binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; transcription factor binding; zinc ion binding
Biological Process: acyl-CoA metabolic process; aging; blood coagulation; cell differentiation; establishment of tissue polarity; glucose homeostasis; intercellular junction assembly and maintenance; intracellular receptor-mediated signaling pathway; lipid homeostasis; lipid metabolic process; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of mitotic cell cycle; negative regulation of protein import into nucleus, translocation; negative regulation of transcription factor activity; negative regulation of tyrosine phosphorylation of Stat5 protein; ornithine metabolic process; phospholipid homeostasis; positive regulation of fatty acid biosynthetic process; positive regulation of gluconeogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of gastrulation; regulation of insulin secretion; regulation of lipid metabolic process; regulation of microvillus biogenesis; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to cAMP; response to drug; response to glucose stimulus; sex differentiation; steroid hormone mediated signaling; transcription from RNA polymerase II promoter; transcription, DNA-dependent; xenobiotic metabolic process
Research Articles on HNF4a
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Product Notes
The Mouse HNF4a hnf4a (Catalog #AAA2533118) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533118 CLIA Kit recognizes Mouse HNF4a. It is sometimes possible for the material contained within the vial of "HNF4a (Hepatocyte Nuclear Factor 4 Alpha), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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