Human LEP (Leptin) CLIA Kit | LEP clia kit

Human LEP (Leptin) CLIA Kit

Cat. Num. AAA2532681

• Gene Names: LEP; OB; OBS; LEPD
• Reactivity: Human
• Synonyms: LEP (Leptin); Human LEP (Leptin) CLIA Kit; LEPD; OB; OBS; Obesity Homolog; LEP clia kit

• Reactivity: Human
• Specificity: This kit recognizes Human LEP in samples. No significant cross-reactivity or interference between Human LEP and analogues was observed.
• Sequence Length: 167

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 18.75pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human LEP were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human LEP were tested on 3 different plates, 20 replicates in each plate.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LEP clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of Human LEP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LEP. You can calculate the concentration of Human LEP in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557715
• NCBI GeneID: 3952
• NCBI Accession #: NP_000221.1
• NCBI GenBank Nucleotide #: NM_000230.2
• UniProt Accession #: P41159; O15158; Q56A88
• Molecular Weight: 18,641 Da
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: LEP
• NCBI Official Synonym Symbols: OB; OBS; LEPD
• NCBI Protein Information: leptin
• UniProt Protein Name: Leptin
• Protein Family: Leptin
• UniProt Gene Name: LEP
• UniProt Synonym Gene Names: OB; OBS
• UniProt Entry Name: LEP_HUMAN

NCBI Description

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

Uniprot Description

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Secreted, signal peptide; Secreted; Hormone; Cell development/differentiation

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; peptide hormone receptor binding; hormone activity

Biological Process: circadian rhythm; response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; regulation of steroid biosynthetic process; female pregnancy; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of appetite; positive regulation of luteinizing hormone secretion; positive regulation of tyrosine phosphorylation of Stat3 protein; response to insulin stimulus; response to vitamin E; positive regulation of MAPKKK cascade; regulation of cholesterol absorption; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; central nervous system neuron development; placenta development; positive regulation of cytokine production; cholesterol metabolic process; positive regulation of developmental growth; bile acid metabolic process; eating behavior; glucose metabolic process; adult feeding behavior; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; glycerol biosynthetic process; insulin secretion; energy reserve metabolic process; response to hypoxia; hormone metabolic process; regulation of gluconeogenesis; positive regulation of follicle-stimulating hormone secretion; positive regulation of insulin receptor signaling pathway; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis

Disease: Leptin Deficiency