Human MT (Melatonin) CLIA Kit | MT clia kit

Human MT (Melatonin) CLIA Kit

Cat. Num. AAA2532577

• Reactivity: Human
• Synonyms: MT (Melatonin); Human MT (Melatonin) CLIA Kit; N-Acetyl-5-Methoxytryptamine; MT clia kit

• Reactivity: Human

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Competitive
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MT were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MT were tested on 3 different plates, 20 replicates in each plate, respectively.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MT clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of Human MT concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This CLIA kit uses the Competitive-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with Human MT. During the reaction, Human MT in samples or Standard competes with a fixed amount of Human MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human MT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The concentration of Human MT in the samples is then determined by comparing the RLU value of the samples to the standard curve.