Cell Division Cycle Protein 42 Recombinant Protein | CDC42 recombinant protein

Recombinant Cell Division Cycle Protein 42 (CDC42)

Cat. Num. AAA2030269

• Gene Names: CDC42; G25K; CDC42Hs
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cell Division Cycle Protein 42; Recombinant Cell Division Cycle Protein 42 (CDC42); CDC42 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEPYTLGLFDTAG QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPEITHHCPKTPF LLVGTQIDLR DDPSTIEKLA KNKQKPITPE TAEKLARDLKAVKYVECSAL TQKGLKNVFD EAILAALEPP EPKKSRRC
• Sequence Length: 191

• Applicable Applications for CDC42 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 24.6kDa
• Accurate Molecular Mass (KD): 27kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Cys188
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 89903012
• NCBI GeneID: 998
• NCBI Accession #: NP_001034891.1
• NCBI GenBank Nucleotide #: NM_001039802.1
• Molecular Weight: 21,311 Da
• NCBI Official Full Name: cell division control protein 42 homolog isoform 1
• NCBI Official Synonym Full Names: cell division cycle 42
• NCBI Official Symbol: CDC42
• NCBI Official Synonym Symbols: G25K; CDC42Hs
• NCBI Protein Information: cell division control protein 42 homolog
• UniProt Protein Name: Cell division control protein 42 homolog
• Protein Family: Cdc42
• UniProt Gene Name: CDC42
• UniProt Entry Name: CDC42_HUMAN

NCBI Description

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]

Uniprot Description

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.