Typical Testing Data/Standard Curve (for reference only)
Rat DAT (Dopamine Transporter) CLIA Kit | DAT clia kit
Rat DAT (Dopamine Transporter) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to DAT. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DAT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DAT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of DAT. You can calculate the concentration of DAT in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]
Uniprot Description
DAT: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI). It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter
Chromosomal Location of Human Ortholog: 5p15.3
Cellular Component: cell surface; cell soma; axon; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; flotillin complex
Molecular Function: protein binding; monoamine transmembrane transporter activity; dopamine binding; protein N-terminus binding; dopamine:sodium symporter activity; dopamine transmembrane transporter activity
Biological Process: lactation; dopamine transport; prepulse inhibition; dopamine catabolic process; monoamine transport; sensory perception of smell; regulation of dopamine metabolic process; locomotory behavior; positive regulation of multicellular organism growth; dopamine biosynthetic process; response to cocaine; synaptic transmission; adenohypophysis development; dopamine uptake; transmembrane transport; neurotransmitter biosynthetic process
Disease: Parkinsonism-dystonia, Infantile; Tobacco Addiction, Susceptibility To
Research Articles on DAT
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Product Notes
The Rat DAT slc6a3 (Catalog #AAA2530843) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2530843 CLIA Kit recognizes Rat DAT. It is sometimes possible for the material contained within the vial of "DAT (Dopamine Transporter), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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