Rabbit MCM3 Polyclonal Antibody | anti-MCM3 antibody

MCM3 Antibody

Cat. Num. AAA2528612

• Gene Names: mcm3; xmcm3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antigen affinity purification
• Synonyms: MCM3; Polyclonal Antibody; MCM3 Antibody; HCC5; MGC1157; P1-MCM3; P1.h; RLFB; anti-MCM3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 807

• Applicable Applications for anti-MCM3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Recombinant protein of human MCM3
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9849 / sc-9850
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MCM3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using MCM3 antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using MCM3 antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using MCM3 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-MCM3 antibody

The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3, 9).

NCBI and Uniprot Product Information

• NCBI GI #: 147903759
• NCBI GeneID: 397821
• NCBI Accession #: NP_001081412.1
• NCBI GenBank Nucleotide #: NM_001087943.1
• Molecular Weight: 90,405 Da
• NCBI Official Full Name: maternal DNA replication licensing factor mcm3
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 3<
• NCBI Official Symbol: mcm3
• NCBI Official Synonym Symbols: xmcm3
• NCBI Protein Information: maternal DNA replication licensing factor mcm3; P1 homolog; XRLF subunit beta; mMCM3; maternal Mcm3 protein; maternal minichromosome maintenance protein 3; p100
• UniProt Protein Name: Maternal DNA replication licensing factor mcm3
• Protein Family: DNA replication licensing factor
• UniProt Gene Name: mmcm3
• UniProt Synonym Gene Names: mMCM3; xMCM3
• UniProt Entry Name: MCM3M_XENLA

Uniprot Description

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. The existence of maternal and zygotic forms of mcm3 and mcm6 suggests that specific forms of mcm2-7 complexes may be used during different stages of development.