Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DDX58 antibody.)
Rabbit anti-Human DDX58 Polyclonal Antibody | anti-DDX58 antibody
DDX58 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen affinity purification
Synonyms
DDX58; Polyclonal Antibody; DDX58 Antibody; DKFZp434J1111; DKFZp686N19181; FLJ13599; RIG-I; anti-DDX58 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-DDX58 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Immunogen
A synthetic peptide of human DDX58
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-48929 / sc-48932 / sc-98911
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DDX58 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DDX58 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human rectal cancer using DDX58 antibody at dilution of 1:200 (200x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human rectal cancer using DDX58 antibody at dilution of 1:200 (200x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cell using DDX58 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cell using DDX58 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-DDX58 antibody
Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes (1-3). TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i. e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection (4, 5). Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5'-triphosphate end not seen in host RNA (8, 9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10, 11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-kappaB, TBK1/IKKepsilon, and IRF-3/IRF-7 (12-15).
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Product Notes
The DDX58 (Catalog #AAA2525273) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDX58 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDX58 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the DDX58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX58, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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