Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human CGMP-dependent protein kinase 1 (PRKG1) Standard Curve.)
Human CGMP-Dependent Protein Kinase 1 (PRKG1) ELISA Kit | PRKG1 elisa kit
Human CGMP-Dependent Protein Kinase 1 (PRKG1) ELISA Kit
Gene Names
PRKG1; CGKI; cGK 1; cGKI-beta
Reactivity
Human
Synonyms
CGMP-Dependent Protein Kinase 1 (PRKG1); Human CGMP-Dependent Protein Kinase 1 (PRKG1) ELISA Kit; PRKG1; RP11-346D6.1; CGKI; DKFZp686K042; FLJ36117; MGC71944; PGK; PKG; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha; protein kinase; cGMP-dependent; regulatory; type I; beta; PRKG1 elisa kit
Reactivity
Human
Specificity
Human CGMP-dependent protein kinase 1 (PRKG1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKG1. No significant cross-reactivity or interference between Human PRKG1 and analogues was observed.
Sequence Length
671
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human CGMP-dependent protein kinase 1 (PRKG1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human CGMP-dependent protein kinase 1 (PRKG1) Standard Curve.)
Related Product Information for PRKG1 elisa kit
Description: This Human CGMP-dependent protein kinase 1 (PRKG1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKG1 in samples. An antibody specific for PRKG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRKG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Two main forms of cGK have been identified: a soluble form designated type I and an intrinsic membrane-bound form designated type II. Prkg1 knockout mice showed impaired platelet responses to VWF or low doses of thrombin and prolonged bleeding time. Human platelet aggregation induced by VWF or low-dose thrombin was inhibited by PRKG1 inhibitors but enhanced by cGMP. Furthermore, a cGMP-enhancing agent, sildenafil, promoted VWF- or thrombin-induced platelet aggregation. The cGMP-stimulated platelet responses were biphasic, consisting of an initial transient stimulatory response that promoted platelet aggregation and a subsequent inhibitory response that limited the size of thrombi.
Background: Two main forms of cGK have been identified: a soluble form designated type I and an intrinsic membrane-bound form designated type II. Prkg1 knockout mice showed impaired platelet responses to VWF or low doses of thrombin and prolonged bleeding time. Human platelet aggregation induced by VWF or low-dose thrombin was inhibited by PRKG1 inhibitors but enhanced by cGMP. Furthermore, a cGMP-enhancing agent, sildenafil, promoted VWF- or thrombin-induced platelet aggregation. The cGMP-stimulated platelet responses were biphasic, consisting of an initial transient stimulatory response that promoted platelet aggregation and a subsequent inhibitory response that limited the size of thrombi.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cGMP-dependent protein kinase 1
NCBI Official Synonym Full Names
protein kinase cGMP-dependent 1
NCBI Official Symbol
PRKG1
NCBI Official Synonym Symbols
CGKI; cGK 1; cGKI-beta
NCBI Protein Information
cGMP-dependent protein kinase 1
UniProt Protein Name
cGMP-dependent protein kinase 1
Protein Family
UniProt Gene Name
PRKG1
UniProt Synonym Gene Names
PRKG1B; PRKGR1A; PRKGR1B; cGK 1; cGK1; cGKI
UniProt Entry Name
KGP1_BOVIN
Research Articles on PRKG1
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Product Notes
The Human PRKG1 prkg1 (Catalog #AAA9716005) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716005 ELISA Kit recognizes Human PRKG1. It is sometimes possible for the material contained within the vial of "CGMP-Dependent Protein Kinase 1 (PRKG1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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