Rabbit anti-Human TNFRSF10B Polyclonal Antibody | anti-TNFRSF10B antibody

TNFRSF10B Antibody

Cat. Num. AAA2522813

• Gene Names: TNFRSF10B; DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: TNFRSF10B; Polyclonal Antibody; TNFRSF10B Antibody; CD262; DR5; KILLER; KILLER/DR5; TRAIL-R2; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; ZTNFR9; anti-TNFRSF10B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 440

• Applicable Applications for anti-TNFRSF10B antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Recombinant protein of human TNFRSF10B
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7192 / sc-7191
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TNFRSF10B antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using TNFRSF10B antibody at dilution of 1:200 (x400 lens))

Related Product Information for anti-TNFRSF10B antibody

The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1, 2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases. DR5 is a receptor for TNF-related apoptosis inducing ligand (TRAIL), which has been been shown to induce apoptosis in variety of cell types and has been targeted for cancer therapy (1-5). Structurally, DR5 contains an amino-terminal leader cleavage site followed by an extracellular region containing two cysteine-rich repeats, then a central transmembrane domain and a carboxy-terminal death domain. DR5 is expressed in a wide variety of tissues and is transcriptional target for p53 (6-8). It induces apoptosis through a FADD-dependent pathway. Deletion of DR5 leads to resistance in TRAIL-mediated apoptosis as well as an abrogated response to DNA-damaging stimuli (9).

NCBI and Uniprot Product Information

• NCBI GI #: 49456751
• NCBI GeneID: 8795
• UniProt Accession #: Q6FH58; O14763
• Molecular Weight: 47,866 Da
• NCBI Official Full Name: TNFRSF10B
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 10b
• NCBI Official Symbol: TNFRSF10B
• NCBI Official Synonym Symbols: DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 10B; Fas-like protein; TNF receptor superfamily member 10b; TNF-related apoptosis-inducing ligand receptor 2; apoptosis inducing protein TRICK2A/2B; apoptosis inducing receptor TRAIL-R2; cytotoxic TRAIL receptor-2; death domain containing receptor for TRAIL/Apo-2L; death receptor 5; p53-regulated DNA damage-inducible cell death receptor(killer); tumor necrosis factor receptor-like protein ZTNFR9
• UniProt Protein Name: TNFRSF10B protein
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF10B
• UniProt Entry Name: Q6FH58_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

TRAIL-R2: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. Homotrimer. Can interact with TRADD and RIPK1. Regulated by p53/TP53. Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p22-p21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; TRAIL binding; receptor activity

Biological Process: caspase activation; regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; cell surface receptor linked signal transduction; induction of apoptosis via death domain receptors; apoptosis; activation of NF-kappaB-inducing kinase

Disease: Squamous Cell Carcinoma, Head And Neck