Mouse AFGF/FGF1 ELISA Kit | AFGF/FGF1 elisa kit
Mouse AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit
Reactivity
Mouse
Synonyms
AFGF/FGF1; Mouse AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit; AFGF/FGF1 elisa kit
Reactivity
Mouse
Specificity
This kit recognizes natural and recombinantMouseAFGF/FGF1. No significant cross-reactivity or interference between MouseAFGF/FGF1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AFGF/FGF1 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseAFGF/FGF1concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AFGF/FGF1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AFGF/FGF1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AFGF/FGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AFGF/FGF1.You can calculate the concentration of AFGF/FGF1in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AFGF/FGF1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AFGF/FGF1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AFGF/FGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AFGF/FGF1.You can calculate the concentration of AFGF/FGF1in the samples by comparing the OD of the samples to the standard curve.
