Western Blot (WB)
(Western blot analysis of ENDOG Antibody (C-term) in 293,K562,Jurkat,NCI-H460 cell line lysates and mouse kidney tissues lysates(35ug/lane). ENDOG (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse ENDOG Polyclonal Antibody | anti-ENDOG antibody
ENDOG, CT (Endonuclease G, Mitochondrial, Endo G) (AP)
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
ENDOG; Polyclonal Antibody; CT (Endonuclease G; Mitochondrial; Endo G) (AP); anti-ENDOG antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ENDOG. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-ENDOG antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human ENDOG (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ENDOG Antibody (C-term) in 293,K562,Jurkat,NCI-H460 cell line lysates and mouse kidney tissues lysates(35ug/lane). ENDOG (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ENDOG Antibody (C-term) in 293,K562,Jurkat,NCI-H460 cell line lysates and mouse kidney tissues lysates(35ug/lane). ENDOG (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(ENDOG Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ENDOG Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ENDOG antibody
References
1. Noda, T., Apoptosis 14(3), 287-297 (2009). 2. Wu, S.L., J. Biomed. Sci. 16, 6 (2009). 3. Ahn, C.H., APMIS 116(6), 534-537 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35 kDa
NCBI Official Full Name
endonuclease G, mitochondrial
NCBI Official Synonym Full Names
endonuclease G
NCBI Official Symbol
ENDOG
NCBI Protein Information
endonuclease G, mitochondrial; endo G; mitochondrial endonuclease G
UniProt Protein Name
Endonuclease G, mitochondrial
Protein Family
UniProt Gene Name
ENDOG
UniProt Synonym Gene Names
Endo G
UniProt Entry Name
NUCG_HUMAN
NCBI Description
The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq, Jul 2008]
Research Articles on ENDOG
Similar Products
Product Notes
The ENDOG endog (Catalog #AAA6473871) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENDOG, CT (Endonuclease G, Mitochondrial, Endo G) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ENDOG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ENDOG endog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENDOG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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