Monkey TNK2 ELISA Kit | TNK2 elisa kit

Monkey TNK2 (Tyrosine Kinase, Non Receptor 2) ELISA Kit

Cat. Num. AAA2515263

• Gene Names: TNK2; ACK; ACK1; ACK-1; p21cdc42Hs
• Reactivity: Monkey
• Synonyms: TNK2; Monkey TNK2 (Tyrosine Kinase; Non Receptor 2) ELISA Kit; TNK2 elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes natural and recombinant Monkey TNK2. No significant cross-reactivity or interference between Monkey TNK2 and analogues was observed.
• Sequence Length: 528

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for TNK2 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey TNK2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNK2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TNK2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNK2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TNK2. You can calculate the concentration of TNK2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20380414
• NCBI GeneID: 10188
• UniProt Accession #: Q07912; Q6ZMQ0; Q8N6U7; Q96H59
• Molecular Weight: 119,349 Da
• NCBI Official Full Name: TNK2 protein
• NCBI Official Synonym Full Names: tyrosine kinase, non-receptor, 2
• NCBI Official Symbol: TNK2
• NCBI Official Synonym Symbols: ACK; ACK1; ACK-1; p21cdc42Hs
• NCBI Protein Information: activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; tyrosine kinase non-receptor protein 2
• UniProt Protein Name: Activated CDC42 kinase 1
• Protein Family: Activated CDC42 kinase
• UniProt Gene Name: TNK2
• UniProt Synonym Gene Names: ACK1; ACK-1
• UniProt Entry Name: ACK1_HUMAN

NCBI Description

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential . Two differentially spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.11.1; EC 2.7.10.2; Kinase, protein; TK group; Ack family

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: cytoplasmic vesicle membrane; clathrin-coated vesicle; dendrite; coated pit; extrinsic to internal side of plasma membrane; adherens junction; growth cone; membrane; cell soma; axon; cytoplasm; plasma membrane; nucleus; endosome

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; WW domain binding; GTPase inhibitor activity; ATP binding; hormone receptor binding; epidermal growth factor receptor binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cell migration; cell surface receptor linked signal transduction; small GTPase mediated signal transduction; negative regulation of catalytic activity; innate immune response; endocytosis; cell differentiation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation