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Typical Testing Data/Standard Curve (for reference only)

Rat G-6-Pase/G6PT/GSD ELISA Kit | G-6-Pase/G6PT/GSD elisa kit

Rat G-6-Pase/G6PT/GSD (Glucose 6 Phosphate) ELISA Kit

Gene Names
G6PC; G6PT; GSD1; G6PC1; GSD1a
Reactivity
Rat
Synonyms
G-6-Pase/G6PT/GSD; Rat G-6-Pase/G6PT/GSD (Glucose 6 Phosphate) ELISA Kit; G-6-Pase/G6PT/GSD elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat G-6-Pase/G6PT/GSD. No significant cross-reactivity or interference between Rat G-6-Pase/G6PT/GSD and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat G-6-Pase/G6PT/GSD and all the analogues, therefore, cross reaction may still exist.
Sequence Length
176
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
78.125-5000mIU/mL
Sensitivity
Min: 46.875mIU/mL; Max: 5000mIU/mL
Preparation and Storage
Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for G-6-Pase/G6PT/GSD elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of G-6-Pase/G6PT/GSD in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat G-6-Pase/G6PT/GSD. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat G-6-Pase/G6PT/GSD and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat G-6-Pase/G6PT/GSD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat G-6-Pase/G6PT/GSD. You can calculate the concentration of Rat G-6-Pase/G6PT/GSD in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,209 Da
NCBI Official Full Name
glucose-6-phosphatase isoform 2
NCBI Official Synonym Full Names
glucose-6-phosphatase, catalytic subunit
NCBI Official Symbol
G6PC
NCBI Official Synonym Symbols
G6PT; GSD1; G6PC1; GSD1a
NCBI Protein Information
glucose-6-phosphatase; G-6-Pase; G6Pase; G6Pase-alpha; glucose-6-phosphatase alpha
UniProt Protein Name
Glucose-6-phosphatase
UniProt Gene Name
G6PC
UniProt Synonym Gene Names
G6PT; G-6-Pase; G6Pase; G6Pase-alpha
UniProt Entry Name
G6PC_HUMAN

NCBI Description

Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]

Uniprot Description

G6PC: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A). A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. Belongs to the glucose-6-phosphatase family.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; EC 3.1.3.9; Carbohydrate Metabolism - starch and sucrose; Membrane protein, multi-pass; Phosphatase (non-protein); Membrane protein, integral; Endoplasmic reticulum; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: endoplasmic reticulum membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: glucose-6-phosphatase activity; phosphate binding; phosphotransferase activity, alcohol group as acceptor

Biological Process: response to food; steroid metabolic process; glycogen metabolic process; phosphorylated carbohydrate dephosphorylation; glycogen catabolic process; multicellular organism growth; glucose 6-phosphate metabolic process; pathogenesis; glucose transport; glucose homeostasis; gluconeogenesis; cholesterol homeostasis; urate metabolic process; triacylglycerol metabolic process; regulation of gene expression; glucose-6-phosphate transport; hexose transport; carbohydrate metabolic process; transmembrane transport

Disease: Glycogen Storage Disease Ia

Research Articles on G-6-Pase/G6PT/GSD

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Product Notes

The Rat G-6-Pase/G6PT/GSD g6pc (Catalog #AAA2512263) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2512263 ELISA Kit recognizes Rat G-6-Pase/G6PT/GSD. It is sometimes possible for the material contained within the vial of "G-6-Pase/G6PT/GSD, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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