Porcine PLTP ELISA Kit | PLTP elisa kit

Porcine PLTP (Phospholipid Transfer Protein) ELISA Kit

Cat. Num. AAA2511794

• Gene Names: PLTP; BPIFE; HDLCQ9
• Reactivity: Porcine
• Synonyms: PLTP; Porcine PLTP (Phospholipid Transfer Protein) ELISA Kit; PLTP elisa kit

• Reactivity: Porcine
• Specificity: This kit recognizes natural and recombinant Porcine PLTP. No significant cross-reactivity or interference between Porcine PLTP and analogues was observed.
• Sequence Length: 405

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for PLTP elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine PLTP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PLTP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PLTP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PLTP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PLTP. You can calculate the concentration of PLTP in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 339275810
• NCBI GeneID: 5360
• NCBI Accession #: NP_001229850.1
• NCBI GenBank Nucleotide #: NM_001242921.1
• UniProt Accession #: P55058; Q8WTT1; Q9BR07; Q9BSH8; A8K006; B4DDD5; B4DRB4; E1P5N8; E7EV16
• Molecular Weight: 44,847 Da
• NCBI Official Full Name: phospholipid transfer protein isoform d
• NCBI Official Synonym Full Names: phospholipid transfer protein
• NCBI Official Symbol: PLTP
• NCBI Official Synonym Symbols: BPIFE; HDLCQ9
• NCBI Protein Information: phospholipid transfer protein; BPI fold containing family E; lipid transfer protein II
• UniProt Protein Name: Phospholipid transfer protein
• Protein Family: Phospholipid transfer protein
• UniProt Gene Name: PLTP
• UniProt Entry Name: PLTP_HUMAN

NCBI Description

The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PLTP: Converts HDL into larger and smaller particles. May play a key role in extracellular phospholipid transport and modulation of hdl particles. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space; extracellular region

Molecular Function: lipid binding

Biological Process: sperm motility; vitamin E biosynthetic process; lipid metabolic process; lipid transport